Engineered endophytic bacteria improve phytoremediation of water-soluble, volatile, organic pollutants
Engineered endophytic bacteria improve phytoremediation of water-soluble, volatile, organic pollutants
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DOI：
10.1038/nbt960
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发表时间：
2004-05-01
影响因子：
46.9
通讯作者：
van der Lelie, D
中科院分区：
文献类型：
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作者：
Barac, T;Taghavi, S;van der Lelie, D
高水溶性和挥发性有机异生物质的植物修复往往是低效的，因为植物不能完全降解这些化合物通过其根际。这导致植物毒性和/或化学品通过叶子挥发，这可能导致额外的环境问题。我们证明，内生细菌配备了适当的降解途径，提高在植物降解甲苯。我们将洋葱伯克霍尔德氏菌G4的pTOM甲苯降解质粒导入B。洋葱属S. 2.4，一种天然的黄羽扇豆内生菌。将该重组菌接种于表面灭菌的羽扇豆种子后，发现该工程菌对甲苯有较强的降解作用，显著降低了甲苯的药害，并使其通过叶片的蒸散量减少了50-70%。这一策略有望提高植物修复挥发性有机污染物的效率。
Phytoremediation of highly water soluble and volatile organic xenobiotics is often inefficient because plants do not completely degrade these compounds through their rhizospheres. This results in phytotoxicity and/or volatilization of chemicals through the leaves, which can cause additional environmental problems. We demonstrate that endophytic bacteria equipped with the appropriate degradation pathway improve the in planta degradation of toluene. We introduced the pTOM toluene-degradation plasmid of Burkholderia cepacia G4 into B. cepacia L. S. 2.4, a natural endophyte of yellow lupine. After surface-sterilized lupine seeds were successfully inoculated with the recombinant strain, the engineered endophytic bacteria strongly degraded toluene, resulting in a marked decrease in its phytotoxicity, and a 50-70% reduction of its evapotranspiration through the leaves. This strategy promises to improve the efficiency of phytoremediating volatile organic contaminants.