Surface-enhanced Raman spectroscopy (SERS) for sub-micromolar detection of DNA/RNA mononucleotides
Surface-enhanced Raman spectroscopy (SERS) for sub-micromolar detection of DNA/RNA mononucleotides
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DOI：
10.1021/ja066263w
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发表时间：
2006-12-13
影响因子：
15
通讯作者：
Sirimuthu, Narayana M. S.
中科院分区：
文献类型：
--
作者：
Bell, Steven E. J.;Sirimuthu, Narayana M. S.
表面增强拉曼（SER）光谱的所有的DNA/RNA单核苷酸已获得高灵敏度使用柠檬酸还原银胶体与MgSO 4聚集，而不是更常见的卤素离子，这被发现，以防止这些化合物的增强。记录腺嘌呤、鸟嘌呤、胸腺嘧啶、胞嘧啶和尿嘧啶沿着相应核苷和5 '-脱氧核苷酸的Sers光谱。对于胞嘧啶系列，所有三个光谱具有相似的相对谱带强度，但腺嘌呤的光谱与腺苷和dAMP的光谱不同，这可能是由于表面上的取向差异。未观察到磷酸盐或糖组的增强条带。嘌呤单核苷酸和嘧啶单核苷酸的Sers光谱之间有一般的相似之处，但光谱有足够的不同，使它们中的每一个被区分开来。因此，该方法可用于高灵敏度，无标记鉴定monoclutenides。
Surface-enhanced Raman (SER) spectra of all the DNA/RNA mononucleotides have been obtained with high sensitivity using citrate-reduced silver colloids aggregated with MgSO4, rather than the more usual halide ions, which were found to prevent enhancement of these compounds. The SERS spectra of adenine, guanine, thymine, cytosine, and uracil were recorded along with their corresponding nucleosides and 5‘-deoxynucleotides. For the cytosine series, all three spectra had similar relative band intensities but the spectra of adenine were different from those of adenosine and dAMP, probably due to differences in orientation on the surface. No enhanced bands from the phosphate or sugar groups were observed. There were general similarities between the SERS spectra of the purine mononucleotides and the pyrimidine mononucleotides, but the spectra were sufficiently different to allow each of them to be distinguished. This method can therefore be used for high sensitivity, label-free identification of mononucleotides.