Effect of diet and exercise on serum insulin, IGF-I, and IGFBP-1 levels and growth of LNCaP cells in vitro (United States)
Effect of diet and exercise on serum insulin, IGF-I, and IGFBP-1 levels and growth of LNCaP cells in vitro (United States)
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DOI：
10.1023/a:1021911517010
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发表时间：
2002-12-01
影响因子：
2.3
通讯作者：
Aronson, WJ
中科院分区：
文献类型：
--
作者：
Ngo, TH;Barnard, RJ;Aronson, WJ
目的：越来越多的证据表明前列腺癌与高水平的血清IGF-I有关。本研究旨在确定低脂饮食和运动(DE)干预是否可以调节IGF轴并减少前列腺癌细胞的体外生长。方法：空腹血清来自14名参加11天DE计划的男性(年龄60+/-3岁)和8名长期遵循DE计划14.2+/-1.7年的相似年龄男性。用酶联免疫吸附试验检测胰岛素、胰岛素样生长因子-I、胰岛素样生长因子结合蛋白-1和胰岛素样生长因子结合蛋白-3，并用血清刺激体外培养的LNCaP细胞生长。结果：血清胰岛素样生长因子-I水平下降20%，胰岛素样生长因子结合蛋白-1升高53%。在长期组，IGF-I比基线低55%，而IGFBP-1比基线高150%。与基线相比，血清胰岛素在11天DE后下降了25%，长期组下降了68%。血清IGFBP-3无明显变化。与基线相比，血清刺激的LNCaP细胞生长在11天后的血清中减少了30%，在长期血清中减少了44%。结论：低脂饮食和运动干预可在体内诱导循环IGF轴的改变，并与体外培养的LNCaP肿瘤细胞生长抑制和凋亡/坏死增加有关。
Objective: Accumulating evidence indicates that prostate cancer is associated with high levels of serum IGF-I. This study was conducted to determine whether a low-fat diet and exercise (DE) intervention may modulate the IGF axis and reduce prostate cancer cell growth in vitro.Methods: Fasting serum was obtained from 14 men (age 60 +/- 3 years) participating in an 11-day DE program and from eight similarly aged men who had followed the DE program for 14.2 +/- 1.7 years (long-term). Insulin, IGF-I, IGFBP-1, and IGFBP-3 were measured by ELISA, and serum was used to stimulate LNCaP cell growth in vitro.Results: Serum IGF-I levels decreased by 20% while IGFBP-1 increased by 53% after 11-day DE. In the long-term group, IGF-I was 55% lower, while IGFBP-1 was 150% higher relative to baseline. Serum insulin decreased by 25% after 11-day DE and was 68% lower in the long-term group, relative to baseline. No changes in serum IGFBP-3 were observed. Serum-stimulated LNCaP cell growth was reduced by 30% in post-11-day serum and by 44% in long-term serum relative to baseline. LNCaP cells incubated with post-DE serum showed increased apoptosis/necrosis, compared to baseline.Conclusions: A low-fat diet and exercise intervention induces in-vivo changes in the circulating IGF axis and is associated with reduced growth and enhanced apoptosis/necrosis of LNCaP tumor cells in vitro.