Polystyrene Spheres-Assisted Matrix-Assisted Laser Desorption Ionization Mass Spectrometry for Quantitative Analysis of Plasma Lysophosphatidylcholines
Polystyrene Spheres-Assisted Matrix-Assisted Laser Desorption Ionization Mass Spectrometry for Quantitative Analysis of Plasma Lysophosphatidylcholines
复制标题
聚苯乙烯球辅助基质辅助激光解吸电离质谱法定量分析血浆溶血磷脂酰胆碱
DOI：
10.1021/ac400452k
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发表时间：
2013-05-07
影响因子：
7.4
通讯作者：
Zhao, Zhenwen
中科院分区：
文献类型：
--
作者：
Wei, Yanbo;Li, Shumu;Zhao, Zhenwen
基质辅助激光解吸电离质谱（MALDI MS）的定量分析是一个挑战，由于来自基质分析物晶体的异质性的重现性差。聚苯乙烯（PS）胶体微球具有上级单分散性，可自组装形成光子晶体。在PS微球的辅助下，构建了一种均一的基质-分析物共晶体，用于血浆溶血磷脂酰胆碱（LPC）的定量分析。对MALDI质谱分析中的基质和溶剂进行了优化，并对重现性和准确度进行了详细考察。本文首次报道了PS球辅助MALDI-MS进行定量分析的简便方法，相信这一方法将大大扩展MALDI-MS的应用范围。
The quantitative analysis by matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) is a challenge due to the poor reproducibility originating from the heterogeneity of the matrix-analyte crystals. Polystyrene (PS) colloidal spheres have superior monodispersed property and can self-assemble to form photonic crystals. With the assistance of PS spheres, a uniform matrix-analyte cocrystal was constructed for the quantitative analysis of plasma lysophosphatidylcholines (LPCs). The matrix and the solvent in MALDI MS analysis were optimized, and the reproducibility and the accuracy were investigated in detail. This is the first report about the very simple method of PS spheres assisted MALDI MS for quantitative analysis, where it is believed that this approach will greatly expand the applications of MALDI MS.