Late expression of a beta chemokine homolog by murine cytomegalovirus.
Late expression of a beta chemokine homolog by murine cytomegalovirus.
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鼠巨细胞病毒晚期表达β趋化因子同系物。
DOI：
10.1128/jvi.71.2.1671-1678.1997
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Virgin4th,HW
中科院分区：
文献类型：
--
作者：
MacDonald,MR;Li,XY;Virgin4th,HW
鼠巨细胞病毒 (MCMV) 中存在的基因 (HJ1) 编码开放阅读框 (ORF)，其预测的氨基酸序列显示与称为趋化因子的 β（或 C-C）类趋化细胞因子同源。同源区位于 116 个氨基酸 ORF 的羧基末端部分。与 HJ1 基因相对应的 0.9-kb RNA 转录本在 MCMV 感染的成纤维细胞和巨噬细胞中表达，作为病毒编码转录本的晚期动力学类别的成员。转录起始位点位于 ORF 中的第三个和第四个蛋氨酸残基之间，预测从第四个蛋氨酸残基开始的一级氨基酸结构，其中包括可能的信号肽以及哺乳动物 β 趋化因子典型的保守半胱氨酸残基。 RNA 转录物是聚腺苷酸化的，表明它可以在 MCMV 感染细胞的细胞质内进行翻译。我们推测，趋化因子同源物在 MCMV 复制周期后期的表达在 MCMV 发病机制中发挥作用，可能是通过作为趋化激动剂或拮抗剂的作用，或者通过改变易感细胞（如巨噬细胞）的激活或分化状态。
A gene (HJ1) present in murine cytomegalovirus (MCMV) encodes an open reading frame (ORF) whose predicted amino acid sequence shows homology to the beta (or C-C) class of chemotactic cytokines known as chemokines. The region of homology is located in the carboxyl-terminal portion of the 116-amino-acid ORF. A 0.9-kb RNA transcript corresponding to the HJ1 gene is expressed in MCMV-infected fibroblasts and macrophages as a member of the late kinetic class of virally encoded transcripts. A transcriptional start site is located between the third and fourth methionine residues in the ORF, predicting a primary amino acid structure, starting at the fourth methionine residue, which includes a possible signal peptide as well as conserved cysteine residues typical for mammalian beta chemokines. The RNA transcript is polyadenylated, suggesting that it can undergo translation within the cytoplasm of an MCMV-infected cell. We speculate that expression of the chemokine homolog at late times in the MCMV replication cycle plays a role in MCMV pathogenesis, possibly by action as a chemotactic agonist or antagonist or by alteration of the activation or differentiation state of a susceptible cell such as a macrophage.