MUTATIONS DEFINING FUNCTIONAL REGIONS OF THE SUPERANTIGEN STAPHYLOCOCCAL ENTEROTOXIN-B
MUTATIONS DEFINING FUNCTIONAL REGIONS OF THE SUPERANTIGEN STAPHYLOCOCCAL ENTEROTOXIN-B
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DOI：
10.1084/jem.175.2.387
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发表时间：
1992-02-01
影响因子：
15.3
通讯作者：
MARRACK, P
中科院分区：
文献类型：
--
作者：
KAPPLER, JW;HERMAN, A;MARRACK, P
葡萄球菌肠毒素B（SE B）既是一种超抗原，又是一种毒素。作为超抗原，SEB可以结合主要组织相容性复合体（MHC）11类分子，以形成携带特定V-β元件的α/β T细胞受体的配体。作为一种毒素，SEB会导致小鼠体重迅速减轻，有时会导致死亡。我们显示，在这里，这两个功能映射到NH 2-末端部分的毒素。确定了三个区域：一个在MHC II类结合中很重要，一个在T细胞识别中，一个在两种功能中。这些结果支持SEB的毒性与大量T细胞刺激和细胞因子介体的释放有关的结论，并表明与MHC和T细胞受体相互作用的残基相互交织。
Staphylococcal enterotoxin B (SEB) is both a superantigen and toxin. As a superantigen, SEB can bind to major histocompatibility complex (MHC) class 11 molecules to form a ligand for alpha/beta T cell receptors bearing particular V-beta elements. As a toxin, SEB causes rapid weight loss in mice sometimes leading to death. We show, here that both of these functions map to the NH2-terminal portion of the toxin. Three regions were identified: one important in MHC class II binding, one in T cell recognition, and one in both functions. These results support the conclusion that the toxicity of SEB is related to massive T cell stimulation and release of cytokine mediators and show that the residues interacting with MHC and the T cell receptor are intertwined.