Purification of rat heart and rat liver citrate synthases. Physical, kinetic, and immunological studies.
Purification of rat heart and rat liver citrate synthases. Physical, kinetic, and immunological studies.
复制标题
大鼠心脏和大鼠肝脏柠檬酸合酶的纯化。
DOI：
10.1016/s0021-9258(18)62217-3
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发表时间：
1971
期刊：
影响因子：
--
通讯作者：
P. Srere
中科院分区：
文献类型：
--
作者：
T. Moriyama;P. Srere
从大鼠心脏和肝脏中分离纯化了柠檬酸合成酶。这些均质蛋白的分子量约为1 × 105，由两个明显相同的亚基组成。乙酰辅酶A和草酰乙酸的动力学常数以及两种酶的kifor ATP的动力学常数相同。利用大鼠心脏酶抗体进行的免疫学研究也表明，这两种酶是相同的。
The citrate synthases from rat heart and rat liver have been purified. These homogeneous proteins have a molecular weight of about 1 x 105and are composed of 2 apparently identical subunits. Kinetic constants for acetyl coenzyme A and oxaloacetate and theKifor ATP are the same for both enzymes. Immunological studies using antibody to the rat heart enzyme also indicate that the enzymes are identical.