Acoustic and current-pulse responses of identified neurons in the dorsal cochlear nucleus of unanesthetized, decerebrate gerbils
Acoustic and current-pulse responses of identified neurons in the dorsal cochlear nucleus of unanesthetized, decerebrate gerbils
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DOI：
10.1152/jn.1999.82.6.3434
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发表时间：
1999-12-01
影响因子：
2.5
通讯作者：
Voigt, HF
中科院分区：
文献类型：
--
作者：
Ding, J;Benson, TE;Voigt, HF
为了建立耳蜗背核(DCN)细胞生理和形态之间的关系，用辣根过氧化物酶(HRP)或神经生物素填充的微管在去脑沙土鼠上进行了细胞内单位记录和标记实验。记录细胞对声(音调和宽带噪声爆发)和电流脉冲刺激的反应，并与细胞形态相关。根据响应图方案(类型I到类型V)对单元进行分类。对已鉴定的19个神经元，包括13个梭形细胞、2个巨细胞和4个车轮细胞的结果表明，这些神经元的细胞形态与其声学反应之间存在相关性。大多数梭形细胞(8/13)与III型单位反应特性有关。梭形细胞的一个子集是I/III型单位(2)、III型单位(2)和TV-T型单位。巨细胞与IV-I型单位反应特性有关。侧翻细胞都有微弱的声学反应，很难分类。膜特性的某些指标也与细胞形态有关，但程度较小。巨细胞和除一个细胞外的所有梭形细胞只发出简单动作电位(AP)，而所有的车轮细胞都发出复杂的AP。巨细胞和梭形细胞都有单调的速率-电流水平曲线，而车轮细胞都有非单调的曲线。这意味着，抑制性声学反应，导致非单调的速率-声级曲线，是由于局部抑制相互作用，而不是严格的膜特性。一个具有III型单位属性的复合峰的梭形细胞表明，车轮细胞不是DCN中唯一的复合峰细胞。DCN梭形细胞的不同反应特性表明，它们对它们接收的兴奋性和抑制性输入的特定补充非常敏感。
In an effort to establish relationships between cell physiology and morphology in the dorsal cochlear nucleus (DCN), intracellular single-unit recording and marking experiments were conducted on decerebrate gerbils using horseradish peroxidase (HRP)- or neurobiotin-filled micropipettes. Intracellular responses to acoustic (tone and broadband noise bursts) and electric current-pulse stimuli were recorded and associated with cell morphology. Units were classified according to the response map scheme (type I to type V). Results from 19 identified neurons, including 13 fusiform cells, 2 giant cells, and 4 cartwheel cells, reveal correlations between cell morphology of these neurons and their acoustic responses. Most fusiform cells (8/13) are associated with type III unit response properties. A subset of fusiform cells was type I/III units (2), type III-i units (2), and a type TV-T unit. The giant cells were associated with type IV-i unit response properties. Cartwheel cells all had weak acoustic responses that were difficult to classify. Some measures of membrane properties also were correlated with cell morphology but to a lesser degree. Giant cells and all but one fusiform cell fired only simple action potentials (APs), whereas all cartwheel cells discharged complex APs. Giant and fusiform cells all had monotonic rate versus current level curves, whereas cartwheel cells had nonmonotonic curves. This implies that inhibitory acoustic responses, resulting in nonmonotonic rate versus sound level curves, are due to local inhibitory interactions rather than strictly to membrane properties. A complex-spiking fusiform cell with type III unit properties suggests that cartwheel cells are not the only complex-spiking cells in DCN. The diverse response properties of the DCN's fusiform cells suggests that they are very sensitive to the specific complement of excitatory and inhibitory inputs they receive.