Phage-based platforms for the clinical detection of human bacterial pathogens.
Phage-based platforms for the clinical detection of human bacterial pathogens.
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DOI：
10.4161/bact.19274
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发表时间：
2012-04-01
期刊：
影响因子：
--
通讯作者：
Westwater C
中科院分区：
文献类型：
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作者：
Schofield DA;Sharp NJ;Westwater C
噬菌体（Bacteriophage，简称噬菌体）作为一种独特的鉴定目标细菌的手段已经使用了几十年。由于其固有的天然特异性、易用性和直接生产，噬菌体具有许多理想的属性，这使得它们特别适合作为细菌检测器。因此，已经对噬菌体或噬菌体衍生产品的开发进行了广泛的研究，以加快人类病原体的检测。然而，很少有基于噬菌体的诊断从研究实验室转变为临床诊断工具。本文综述了目前用于结核分枝杆菌、鼠疫耶尔森菌、炭疽杆菌和金黄色葡萄球菌检测的噬菌体平台。我们简要介绍了疾病，目前的诊断选择，以及噬菌体诊断在确定感染原因和确定抗生素敏感性方面的作用。
Bacteriophages (phages) have been utilized for decades as a means for uniquely identifying their target bacteria. Due to their inherent natural specificity, ease of use, and straightforward production, phage possess a number of desirable attributes which makes them particularly suited as bacterial detectors. As a result, extensive research has been conducted into the development of phage, or phage-derived products to expedite the detection of human pathogens. However, very few phage-based diagnostics have transitioned from the research lab into a clinical diagnostic tool. Herein we review the phage-based platforms that are currently used for the detection of Mycobacterium tuberculosis, Yersinia pestis, Bacillus anthracis and Staphylococcus aureus in the clinical field. We briefly describe the disease, the current diagnostic options, and the role phage diagnostics play in identifying the cause of infection, and determining antibiotic susceptibility.