PROCEDURE FOR IDENTIFYING NONSENSE MUTATIONS
PROCEDURE FOR IDENTIFYING NONSENSE MUTATIONS
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DOI：
10.1128/jb.96.1.215-220.1968
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发表时间：
1968-01-01
影响因子：
3.2
通讯作者：
AMES, BN
中科院分区：
文献类型：
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作者：
BERKOWIT.D;HUSHON, JM;AMES, BN
设计了一种快速鉴定沙门氏菌无义突变（UAG、UAA、UGA密码子）的方法（U，尿苷; A，腺嘌呤; G，鸟嘌呤）。将待测突变回复突变，并将回复突变体复制打印到铺有试验菌株菌苔的乳糖平板上。这些试验菌株含有F[image] lac附加体，附加体Z基因中存在无义突变。用来自试验菌株菌苔的附加体感染回复突变体。因为S.鼠伤寒不能发酵乳糖，只有那些回复突变体。有无义抑制子的细菌能够在乳糖上生长。如果在乳糖平板上出现菌落，则可以得出结论，原始菌株携带无义突变，因为无义抑制子抑制突变表型。
A method was devised for the rapid identification of nonsense mutations (UAG, UAA, UGA codons) in Salmonella (U, uridine; A, adenine, G, guanine). The mutations to be tested are reverted, and the rever-tants are replica-printed onto lactose plates spread with lawns of tester strains. These tester strains contain F[image] lac episomes with nonsense mutations in the episomal Z gene. The revertants are infected with the episome from the tester strain lawn. Because S. typhimurium is unable to ferment lactose, only those revertants. which have nonsense suppressors are able to grow on lactose. If colonies appear on the lactose plate, it may be concluded that the original strain carries a nonsense mutation, since nonsense suppressors suppress the mutant phenotype.