Radiation-induced oxidative DNA damage, 8-oxoguanine, in human peripheral T cells.
Radiation-induced oxidative DNA damage, 8-oxoguanine, in human peripheral T cells.
复制标题
人外周 T 细胞中辐射诱导的氧化 DNA 损伤（8-氧代鸟嘌呤）。
DOI：
10.3892/ijmm.11.1.27
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发表时间：
2003
影响因子：
5.4
通讯作者：
S. Yoshida
中科院分区：
文献类型：
--
作者：
Y. Ogawa;Toshihiro Kobayashi;A. Nishioka;S. Kariya;S. Hamasato;H. Seguchi;S. Yoshida
导致T淋巴细胞高度放射敏感性的机制尚未完全被描述。在我们先前的研究中，我们发现在5Gy射线照射后13小时和24小时，人外周T淋巴细胞分别出现早期的凋亡改变(膜联蛋白V阳性)和晚期的凋亡改变(碘化丙啶阳性)。照射后10h观察线粒体膜电位的变化。随后证实线粒体细胞色素c的释放。为了阐明线粒体膜电位改变之前发生的机制，我们研究了10 mV X射线照射后人外周血T淋巴细胞DNA氧化损伤的剂量和时间。结果表明，DNA氧化损伤产物8-氧鸟嘌呤的产生分别在照射后10、6、3h、2、5、20Gy3小时开始出现。因此，我们得出结论，仍有必要评估辐射诱导的DNA氧化损伤的程度。此外，根据在各种类型的正常组织细胞和肿瘤细胞中发现的辐射敏感性的不同，分析超氧阴离子自由基的产生和清除是重要的。
The mechanism leading to the high level of radiosensitivity of T lymphocytes has not yet been fully described. In our previous study, we demonstrated that human peripheral T lymphocytes revealed early apoptotic changes (annexin V-positive) and late apoptotic changes (propidium iodide-positive), at 13 and 24 h after irradiation of 5 Gy, respectively. Changes in mitochondrial membrane potential were observed at 10 h after irradiation of 5 Gy. Subsequently, mitochondrial cytochrome c release was confirmed. In order to elucidate the mechanism which occurs prior to the mitochondrial membrane potential changes, we examined in the present study the radiation dose and the timing of oxidative DNA damage induced in human peripheral T lymphocytes following 10 MV X-ray irradiation. As a result, the production of 8-oxoguanine, i.e., the product of oxidative DNA damage, was clearly identified starting at 10, 6, and 3 h, after 2, 5, and 20 Gy of irradiation, respectively. Therefore, we concluded that it remains necessary to evaluate the extent of radiation-induced oxidative DNA damage. Furthermore, it is important to analyze superoxide radical production and scavenging in terms of the variety of radiosensitivities found among various types of normal tissue cells and neoplastic cells.