Lysyl oxidase secreted by tumour endothelial cells promotes angiogenesis and metastasis.
Lysyl oxidase secreted by tumour endothelial cells promotes angiogenesis and metastasis.
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DOI：
10.1038/bjc.2013.535
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发表时间：
2013-10-15
影响因子：
8.8
通讯作者：
Hida, K.
中科院分区：
文献类型：
--
作者：
Osawa, T.;Ohga, N.;Akiyama, K.;Hida, Y.;Kitayama, K.;Kawamoto, T.;Yamamoto, K.;Maishi, N.;Kondoh, M.;Onodera, Y.;Fujie, M.;Shinohara, N.;Nonomura, K.;Shindoh, M.;Hida, K.
在肿瘤内皮细胞(TECs)中高表达的分子可能是特异性靶向TEC的候选分子。通过DNA芯片分析发现，与正常内皮细胞(NECs)相比，赖氨酰氧化酶(LOX)基因在TECs中的表达上调。LOX是一种增强肿瘤细胞侵袭和转移的酶。然而，关于LOX在分离的TEC中的作用还没有报道。分离TECs和NECs，研究TECs的LOX功能。用β-氨基丙腈评价LOX对体内肿瘤生长的抑制作用。LOX在TECs的表达高于NECs。LOX基因敲除抑制了TECs的细胞迁移和管状形成，这与粘着斑激酶(Tyr397)的磷酸化减少有关。免疫组织化学染色显示LOX在体内人肿瘤血管中高表达。在体内肿瘤模型中，BAPN可抑制肿瘤血管生成和微转移。LOX可能是TEC的标志物，也可能成为抗血管生成治疗的新靶点。
Molecules that are highly expressed in tumour endothelial cells (TECs) may be candidates for specifically targeting TECs. Using DNA microarray analysis, we found that the lysyl oxidase (LOX) gene was upregulated in TECs compared with its expression in normal endothelial cells (NECs). LOX is an enzyme that enhances invasion and metastasis of tumour cells. However, there are no reports on the function of LOX in isolated TECs. TECs and NECs were isolated to investigate LOX function in TECs. LOX inhibition of in vivo tumour growth was also assessed using β-aminopropionitrile (BAPN). LOX expression was higher in TECs than in NECs. LOX knockdown inhibited cell migration and tube formation by TECs, which was associated with decreased phosphorylation of focal adhesion kinase (Tyr 397). Immunostaining showed high LOX expression in human tumour vessels in vivo. Tumour angiogenesis and micrometastasis were inhibited by BAPN in an in vivo tumour model. LOX may be a TEC marker and a possible therapeutic target for novel antiangiogenic therapy.
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影响因子：
5.7
作者：
Ohga, Noritaka;Hida, Kyoko;Shindoh, Masanobu
通讯作者：
Shindoh, Masanobu
影响因子：
11.5
作者：
Hayes, Daniel F.;Cristofanilli, Massimo;Terstappen, Leon W. W. M.
通讯作者：
Terstappen, Leon W. W. M.
影响因子：
3.7
作者：
Bondareva A;Downey CM;Ayres F;Liu W;Boyd SK;Hallgrimsson B;Jirik FR
通讯作者：
Jirik FR
影响因子：
3.7
作者：
Kawamoto T;Ohga N;Akiyama K;Hirata N;Kitahara S;Maishi N;Osawa T;Yamamoto K;Kondoh M;Shindoh M;Hida Y;Hida K
通讯作者：
Hida K
影响因子：
8.8
作者：
Kurosu, T.;Ohga, N.;Hida, Y.;Maishi, N.;Akiyama, K.;Kakuguchi, W.;Kuroshima, T.;Kondo, M.;Akino, T.;Totsuka, Y.;Shindoh, M.;Higashino, F.;Hida, K.
通讯作者：
Hida, K.