Shorter sperm telomere length in association with exposure to polycyclic aromatic hydrocarbons: Results from the MARHCS cohort study in Chongqing, China and in vivo animal experiments
Shorter sperm telomere length in association with exposure to polycyclic aromatic hydrocarbons: Results from the MARHCS cohort study in Chongqing, China and in vivo animal experiments
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精子端粒长度较短与接触多环芳烃有关：中国重庆 MARHCS 队列研究和体内动物实验的结果
DOI：
10.1016/j.envint.2016.08.001
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发表时间：
2016-10-01
影响因子：
11.8
通讯作者：
Cao, Jia
中科院分区：
文献类型：
--
作者：
Ling, Xi;Zhang, Guowei;Cao, Jia
已有研究表明，多环芳烃(PAHs)具有生殖毒性，精子端粒长度缩短可能是导致男性不育的原因之一。然而，暴露于多环芳烃是否与精子端粒长度(STL)相关，从未被评估过。本研究旨在评估PAHs暴露与STL之间的潜在联系，并探索可能预测低水平PAHs暴露对人类精子影响的潜在生物标志物。2014年参加重庆大学生男性生殖健康队列研究的666名志愿者进行了问卷调查和生物样本采集。656名参与者测量了精液参数，492名参与者、199名参与者和628名参与者成功地检测了尿多环芳烃代谢产物、STL和精子凋亡率。线性回归分析显示，尿1-羟基苯(1-OHPYr)和1-羟基萘(1-OHNAP)水平升高与STL降低相关(-0385；1-OHPYR的95%CI为-0.749，-0.021；1-OHNAP的-0.079；-95%CI，-0.146，-0.011)。在调整了潜在的混杂因素后，显著的负面关联仍然存在。然而，尿多环芳烃代谢产物与精液质量或精子凋亡之间没有明显的关联。我们还给大鼠灌服苯并[a]芘(B[a]P；0、1、5和10 mg/kg)4周，发现生殖细胞STL缩短，端粒酶表达减少，并呈剂量依赖关系。综上所述，环境中暴露于某些多环芳烃可能与人类STL降低有关，体内动物实验结果也证实了苯并[a]P对雄性生殖细胞端粒的不利影响。(C)2016爱思唯尔有限公司。保留所有权利。
It has been well demonstrated that polycyclic aromatic hydrocarbons (PAHs) can cause reproductive toxicity, and shorter telomere length in sperm may be one of the factors causing male infertility. However, whether exposure to PAHs is associated with sperm telomere length (STL) has never been evaluated. The present study aimed to assess the potential association between PAHs exposure and STL, and to explore potential biomarkers that may predict the effects of low-level exposure to PAHs on human sperm. Questionnaires and biological samples were collected from 666 volunteers participating in the Male Reproductive Health in Chongqing College Students (MARHCS) cohort study in 2014. Semen parameters were measured for 656 participants, while urinary PAH metabolites, STL and sperm apoptosis were successfully measured for 492,199 and 628 participants, respectively. The linear regression analysis revealed that increased levels of urinary 1-hydroxypyrene (1-OHPyr) and 1-hydroxynapthalene (1-OHNap) were associated with decreased STL (-0385; 95% CI, -0.749, -0.021 for 1-OHPyr; and -0.079; -95% CI, -0.146, -0.011 for 1-OHNap). The significant negative associations remained after adjusting for potential confounders. However, no significant associations were observed between urinary PAH metabolites and semen quality or sperm apoptosis. We also administrated rats with benzo[a]pyrene (B[a]P; 0, 1, 5, and 10 mg/kg) for 4 weeks and found shorter STL and decreased telomerase expression in germ cells in a dose-dependent manner. In conclusion, environmental exposure to some PAHs may be associated with decreased human STL, and the in vivo animal results also demonstrate the adverse effects of B[a]P on telomere of male germ cells. (C) 2016 Elsevier Ltd. All rights reserved.