Sigma factor RpoN employs a dual transcriptional regulation for controlling twitching motility and biofilm formation in Lysobacter enzymogenes OH11
Sigma factor RpoN employs a dual transcriptional regulation for controlling twitching motility and biofilm formation in Lysobacter enzymogenes OH11
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Sigma 因子 RpoN 采用双重转录调控来控制溶杆菌 OH11 的抽搐运动和生物膜形成
DOI：
10.1007/s00294-017-0770-z
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发表时间：
2018-04-01
期刊：
影响因子：
2.5
通讯作者：
Qian, Guoliang
中科院分区：
文献类型：
--
作者：
Han, Sen;Shen, Danyu;Qian, Guoliang
溶杆菌属是一种革兰氏阴性菌属，包括一组环境细菌，其具有产生丰富的新型抗生素的能力，以及采用独特的IV型菌毛（T4P）介导的抽搐运动（TM），其仍然知之甚少。在这里，我们使用L。酶基因OH 11表现出显着的抗真菌活性作为工作模型，以解决这个问题。通过突变菌株OH11中的28个潜在的σ因子，我们已经鉴定了一种蛋白RpoN（OH11）（σ 54），其对于T4P形成和TM是必不可少的。我们进一步发现RpoN（OH11）不仅调节pilA的转录，而且还调节另一个编码杂交双组分转导系统的关键基因chpA。洛杉矶酶基因RpoN（OH11）直接与chpA的启动子结合，控制其转录，这是T4P介导的TM所必需的。据我们所知，这样的转录调控进行的RpoN控制细菌TM从未被报道。最后，我们证明了L.酶基因OH 11也可以产生生物膜，该生物膜可能被该菌株用于感染真菌病原体。rpoN（OH11）、pilA和chpA的突变均导致生物被膜形成显著减少，提示RpoN（OH11）对pilA和chpA的双重转录调控是其调控生物被膜形成的关键。酶基因总之，本研究确定chpA作为RpoN控制T4P介导的抽搐运动和生物膜形成的新靶点。酶基因OH 11。
Lysobacter is a Gram-negative genus comprising a group of environmental bacteria with abilities to produce abundant novel antibiotics, as well as adopting a unique type IV pilus (T4P)-mediated twitching motility (TM) that remains poorly understood. Here, we employ L. enzymogenes OH11 exhibiting significant antifungal activity as a working model to address this issue. Via mutating the 28 potential sigma factors in strain OH11, we have identified one protein RpoN(OH11) (sigma 54) that is indispensable for T4P formation and TM. We further showed that RpoN(OH11) not only regulates the transcription of pilA, but also another crucial gene chpA that encodes a hybrid two-component transduction system. The L. enzymogenes RpoN(OH11) was found to directly bind to the promoter of chpA to control its transcription, which is found to be essential for the T4P-mediated TM. To our knowledge, such a transcriptional regulation performed by RpoN in control of bacterial TM has never been reported. Finally, we showed that L. enzymogenes OH11 could also produce biofilm that is likely employed by this strain to infect fungal pathogens. Mutation of rpoN (OH11), pilA and chpA all led to a significant decrease in biofilm formation, suggesting that the dual transcriptional regulation of pilA and chpA by RpoN(OH11) plays a key role for RpoN(OH11) to modulate the biofilm formation in L. enzymogenes. Overall, this study identified chpA as a new target of RpoN for controlling the T4P-mediated twitching motility and biofilm formation in L. enzymogenes OH11.