Cryomicroscopy of radiation sensitive specimens on unmodified graphene sheets: Reduction of electron-optical effects of charging
Cryomicroscopy of radiation sensitive specimens on unmodified graphene sheets: Reduction of electron-optical effects of charging
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DOI：
10.1016/j.jsb.2013.04.014
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发表时间：
2013-09-01
影响因子：
3
通讯作者：
Rosenthal, Peter B.
中科院分区：
文献类型：
--
作者：
Sader, Kasim;Stopps, Martyn;Rosenthal, Peter B.
电子显微镜获得的对辐射敏感的样品的图像质量下降，超过了仅由于电子束引起的样品充电或移动而造成的辐射损伤的质量下降。对于生物标本，当它们悬浮在玻璃冰的绝缘层中时，充电和移动最严重，否则，这是在接近自然状态下保存水合标本的最佳选择。我们对生物标本进行成像，包括在悬浮的未经修饰的石墨烯薄片上的薄薄的玻璃冰膜中的单颗粒蛋白质复合体和脂膜包裹的病毒。我们表明，在这样的制备中，通过成像光束的电子光学微扰来评估冰的电荷被消除了。我们还使用相同的样品载体在液氮温度下记录在石墨烯上生长的单层石蜡晶体的高分辨率图像。(C)2013年提交人。爱思唯尔公司出版，版权所有。
Images of radiation-sensitive specimens obtained by electron microscopy suffer a reduction in quality beyond that expected from radiation damage alone due to electron beam-induced charging or movement of the specimen. For biological specimens, charging and movement are most severe when they are suspended in an insulating layer of vitreous ice, which is otherwise optimal for preserving hydrated specimens in a near native state. We image biological specimens, including a single particle protein complex and a lipid-enveloped virus in thin, vitreous ice films over suspended sheets of unmodified graphene. We show that in such preparations, the charging of ice, as assessed by electron-optical perturbation of the imaging beam, is eliminated. We also use the same specimen supports to record high resolution images at liquid nitrogen temperature of monolayer paraffin crystals grown over graphene. (C) 2013 The Authors. Published by Elsevier Inc. All rights reserved.