Functional evaluation of marine-derived tocopherol, a minor homolog of vitamin E, on adipocyte differentiation and inflammation using 3T3-L1 and RAW264.7 cells
Functional evaluation of marine-derived tocopherol, a minor homolog of vitamin E, on adipocyte differentiation and inflammation using 3T3-L1 and RAW264.7 cells
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DOI：
10.1007/s12562-020-01404-6
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发表时间：
2020-02-19
影响因子：
1.9
通讯作者：
Gotoh, Naohiro
中科院分区：
文献类型：
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作者：
Beppu, Fumiaki;Aida, Yusuke;Gotoh, Naohiro
海洋衍生的生育酚（MDT）通常存在于栖息于冷水中的海洋生物中，是具有维生素E（VE）活性的单不饱和母育酚。为了评价这种次要VE同系物的功能，我们研究了MDT对小鼠细胞系3 T3-L1脂肪细胞和小鼠单核细胞/巨噬细胞系RAW 264.7细胞中脂肪细胞分化和炎症因子产生的影响。在分化过程中用10和20 μ M MDT处理3 T3-L1脂肪细胞增强了脂质积累，同时上调了过氧化物酶体增殖物激活受体γ和CCAAT/增强子结合蛋白的表达。与对照脂肪细胞相比，MDT处理还增加脂联素的分泌和基因表达，而减少白细胞介素6（IL-6）和单核细胞趋化蛋白-1的分泌和基因表达。MDT治疗导致更高的脂肪生成活性和更高的炎症因子的调节比治疗α和γ-生育酚，主要的同源系列的VE。MDT处理的RAW264.7细胞显示与对照RAW264.7细胞相比显著降低的脂多糖诱导的IL-6和肿瘤坏死因子α分泌。这些结果表明，MDT可能具有预防肥胖相关炎症进展为代谢紊乱的潜在作用。
Marine-derived tocopherol (MDT), which is typically found in marine organisms that inhabit cold water, is a monounsaturated tocol having vitamin E (VE) activity. To evaluate the functionality of this minor VE homolog, we have studied the influence of MDT on adipocyte differentiation and the production of inflammatory factors in mouse cell line 3T3-L1 adipocytes and mouse monocyte/macrophage cell line RAW264.7 cells. Treatment of 3T3-L1 adipocytes with 10 and 20 mu M MDT during differentiation enhanced lipid accumulation and concurrently upregulated peroxisome proliferator activated receptor gamma and CCAAT/enhancer-binding protein expression. Compared to the control adipocytes, MDT treatment also increased the secretion and gene expression of adiponectin, while it decreased the secretion and gene expression of interleukin 6 (IL-6) and monocyte chemoattractant protein-1. Treatment with MDT resulted in higher adipogenesis activity and higher regulation of inflammatory factors than did treatment with alpha- and gamma-tocopherol, the predominant homologous series of VE. MDT-treated RAW264.7 cells showed significantly decreased lipopolysaccharide-induced IL-6 and tumor necrosis factor alpha secretion compared to the control RAW264.7 cells. These results suggest that MDT could have a potential role for preventing obesity-related inflammation progressing to metabolic disorders.