Peptides and Astroglia Improve the Regenerative Capacity of Alginate Gels in the Injured Spinal Cord.
Peptides and Astroglia Improve the Regenerative Capacity of Alginate Gels in the Injured Spinal Cord.
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DOI：
10.1089/ten.tea.2018.0082
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发表时间：
2019-04
影响因子：
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通讯作者：
Thomas Schackel;P. Kumar;M. Günther;Shengwen Liu;Manuel Brunner;Beatrice Sandner;R. Puttagunta;R. M
中科院分区：
文献类型：
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作者：
Thomas Schackel;P. Kumar;M. Günther;Shengwen Liu;Manuel Brunner;Beatrice Sandner;R. Puttagunta;R. M
影响声明跨越受伤脊髓病变的轴突桥需要生长基质和引导线索。使用具有毛细通道的藻酸盐水凝胶，我们发现聚-L-鸟氨酸和层粘连蛋白可以稳定结合，并通过增强受损脊髓中的宿主细胞浸润来改善体外细胞粘附和神经突生长，以及体内轴突生长。用出生后星形胶质细胞填充涂层水凝胶进一步增加了短距离轴突生长，并导致在宿主/移植物界面上形成连续的星形胶质基质。因此，带正电荷的生物活性分子可以稳定地结合到各向异性毛细管藻酸盐水凝胶上，并且早期星形胶质细胞进一步促进组织整合。
IMPACT STATEMENT Axonal bridging across a lesion in the injured spinal cord requires a growth substrate and guidance cues. Using alginate hydrogels with capillary channels we show that poly-l-ornithine and laminin can be stably bound and improve cell adhesion and neurite growth in vitro, and axon growth in vivo by enhancing host cell infiltration in the injured spinal cord. Filling of coated hydrogels with postnatal astrocytes further increases short-distance axon growth and results in a continuous astroglial substrate across the host/graft interface. Thus, positively charged bioactive molecules can be stably bound to anisotropic capillary alginate hydrogels and early astrocytes further promote tissue integration.