Hemin levels in culture medium of Porphyromonas (Bacteroides) gingivalis regulate both hemin binding and trypsinlike protease production
Hemin levels in culture medium of Porphyromonas (Bacteroides) gingivalis regulate both hemin binding and trypsinlike protease production
复制标题
牙龈卟啉单胞菌（拟杆菌）培养基中的血红素水平调节血红素结合和胰蛋白酶样蛋白酶的产生
作者：
R. Carman;M. Ramakrishnan;F. Harper
洗涤细胞和不溶于Sarkosyl的外膜制备牙龈卟啉单胞菌W50结合氯化血红素的黑色素类杆菌。如果细菌生长在每升添加5毫克氯化血红素的肉汤中，则细胞和外膜从缓冲溶液中去除的氯化血红素量明显更大。相反，在没有补充氯化血红素的情况下生长的细胞结合相对较少。然而，所有的制剂都结合了一些氯化血红素。此外，氯化高铁血红素可调节牙龈假单胞菌W50产生显著更高水平的类胰蛋白酶。无色素变异体W50BE1对生长介质中的氯化血红素水平没有表现出这样的反应。
Washed cells and Sarkosyl-insoluble outer membrane preparations of the black-pigmented bacteroides Porphyromonas gingivalis W50 bound hemin. The amount of hemin removed from a buffered solution by both cells and outer membranes was significantly larger if bacteria had been grown in broths supplemented with 5 mg of hemin per liter rather than none. Conversely, cells grown without supplemental hemin bound relatively little. However, all preparations bound some hemin. In addition, hemin regulated the production of significantly higher levels of trypsinlike protease by P. gingivalis W50. The nonpigmented variant, W50 BE1, showed no such responses to the levels of hemin in the growth medium.