Effects of carbon ion beam irradiation on lung injury and pulmonary fibrosis in mice
Effects of carbon ion beam irradiation on lung injury and pulmonary fibrosis in mice
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碳离子束照射对小鼠肺损伤及肺纤维化的影响
DOI：
10.3892/etm.2013.881
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发表时间：
2013-01
影响因子：
2.7
通讯作者：
Liu, Yuanyuan
中科院分区：
文献类型：
--
作者：
Zhang, Zhongxiang;Wang, Xinyu;Yang, Rong;Liu, Yang;Zhao, Weiping;Si, Jin;Ma, Xiaofei;Sun, Chao;Liu, Yuanyuan
放射性肺损伤是核事故、骨髓移植预处理和胸部放射治疗的常见并发症。其发病机制复杂，目前尚无特效治疗方法。为研究胸部肿瘤重离子照射后的放射性肺损伤，昆明种小鼠随机分为4组：正常对照组和兰州重离子研究中心(HIRFL)235 MeV/u~(12)C6+全身照射2、4、6Gy组。照射后1、2、3、4、5、6个月，苏木精-伊红染色观察病理改变，分光光度法检测羟脯氨酸(HP)含量。检测肺组织中肿瘤坏死因子-α和转化生长因子-β的表达。结果表明，与对照组相比，照射后4个月、6个月照射组小鼠肺组织Hp含量明显升高，但4个月后变化不明显。照射后1~4个月Hp含量明显升高，4个月后Hp含量明显下降。肺损伤程度随照射剂量的增加而增加，但随照射时间的延长而减轻。实验组血清肿瘤坏死因子-α水平在2个月时升高，2个月后达最大值，2个月后显著下降。转化生长因子-β的表达在4个月时显著增加，4、6Gy组变化不大，2Gy射线照射组在4个月后明显下降。提示重离子照射对胸部肿瘤有一定程度的肺损伤，而2Gy射线照射对肺的损伤可能较小，为重离子治疗胸部肿瘤提供了科学依据。
Radiation-induced lung injury is a well-described complication of nuclear accidents, marrow-transplant pretreatment and thoracic radiotherapy. The mechanism is complex and no special therapy for it is available at present. To study radiation pulmonary injury following heavy ion radiotherapy for thoracic tumors, Kunming mice were randomly divided into 4 groups: normal control and 2, 4 and 6 Gy irradiation groups which underwent whole-body exposure to 235 MeV/u 12C6+ administered at the Heavy Ion Research Facility in Lanzhou (HIRFL). The pathological changes were observed by hematoxylin and eosin staining and the hydroxyproline (HP) content was assessed by spectrophotometry at months 1, 2, 3, 4, 5 and 6 after radiation exposure. In addition, the expression of tumor necrosis factor (TNF)-α and transforming growth factor (TGF)-β in the lung tissues was measured. The results showed that, compared with the control group, the lung tissue HP content was increased following irradiation but did not statistically significantly change after 4 months in the 4- and 6-Gy-treated groups. However, in the 2-Gy-treated group, the HP content was markedly increased between months 1 and 4 and decreased after month 4. The extent of the lung injury was significantly increased by the higher radiation dosages but was relieved in the 2 Gy group as the time since irradiation increased. The results also revealed that the levels of TNF-α were upregulated and reached a maximum at month 2, but decreased noticeably 2 months later in the experimental groups. The expression of TGF-β increased markedly in month 4 and was altered little in the 4- and 6-Gy-treated groups but decreased sharply in the 2 Gy irradiation group after month 4. These findings suggest that heavy ion radiotherapy for chest tumors causes lung injury to a certain extent, while there is likely to be little injury to lungs treated with <2 Gy, which provides scientific evidence for the use of heavy ion therapy for thoracic tumors.
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DOI：
10.1201/b13341-74
发表时间：
--
期刊：
--
影响因子：
--
作者：
通讯作者：
--
影响因子：
2.6
作者：
Jason S. Zolak;J. D. de Andrade
通讯作者：
Jason S. Zolak;J. D. de Andrade
影响因子：
9
作者：
Haroon, ZA;Raleigh, JA;Dewhirst, MW
通讯作者：
Dewhirst, MW
影响因子：
12.4
作者：
Sergio Harari;A. Caminati
通讯作者：
Sergio Harari;A. Caminati
影响因子：
1.2
作者：
Prasse, A.
通讯作者：
Prasse, A.