LIGHT AND ELECTRON-MICROSCOPY OF DORSAL SPINOCEREBELLAR TRACT NEURONS IN THE CAT - AN INTRACELLULAR HORSERADISH-PEROXIDASE STUDY
LIGHT AND ELECTRON-MICROSCOPY OF DORSAL SPINOCEREBELLAR TRACT NEURONS IN THE CAT - AN INTRACELLULAR HORSERADISH-PEROXIDASE STUDY
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DOI：
10.1113/expphysiol.1983.sp002761
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发表时间：
1983-01-01
影响因子：
--
通讯作者：
BROWN, AG
中科院分区：
文献类型：
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作者：
HOUCHIN, J;MAXWELL, DJ;BROWN, AG
将辣根过氧化物酶细胞内注射到猫克拉克柱的背侧脊髓小脑 DSCT 神经元中。所有 DSCT 神经元均由 I 组肌肉传入纤维兴奋。在光学显微镜和电镜水平上检查染色的神经元，并对光学显微镜材料进行计算机辅助重建和定量分析。 DSCT 神经元的树突树在脊髓长轴上延伸约 3 毫米，并在横向平面上被证实位于克拉克柱内或非常靠近克拉克柱。树突分枝广泛，具有各种不规则性：复杂的细枝簇、脊柱状突起和珠状静脉曲张。这些细胞的轴突都没有显示出任何释放侧支循环的迹象。计算机辅助重建允许从任何角度观察树突树，并能够测量其总树突长度、表面积和体积。根据 Rall 的 3/2 幂法则测试分支点处的树突直径。 EM 分析证实并扩展了之前的研究。含有扁平囊泡的布顿可能位于 DSCT 神经元和与相同 DSCT 树突接触的巨大突触末端的突触前。
Intracellular injections of horseradish peroxidase were made into dorsal spinocerebellar DSCT neurons in Clarke''s column of the cat. All the DSCT neurons were excited from group I muscle afferent fibers. The stained neurons were examined at the light microscopy and EM level, and light microscope material was subjected to computer-aided reconstruction and quantitative analysis. The dendritic trees of DSCT neurones extend about 3 mm in the long axis of the spinal cord and were confirmed, in the transverse plane, within or very close to Clarke''s column. The dendrites branched extensively and carried various irregularities: complex clusters of fine branches, spine-like protuberances and bead-like varicosities. None of the axons of the cells showed any sign that they gave off collaterals. Computer-aided reconstruction allowed the dendritic trees to be viewed from any angle and enabled measurements of their total dendritic lengths, surface area and volume. The dendritic diameters at branch points were tested against Rall''s 3/2 power rule. EM analysis confirmed and extended previous studies. Boutons containing flattened vesicles could be presynaptic to both a DSCT neuron and to a giant synaptic terminal that made contact with same DSCT dendrite.