A DNAβ associated with Tomato Yellow Leaf Curl China Virus is required for symptom induction
A DNAβ associated with Tomato Yellow Leaf Curl China Virus is required for symptom induction
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DOI：
10.1128/jvi.78.24.13966-13974.2004
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发表时间：
2004-12-01
影响因子：
5.4
通讯作者：
Zhou, XP
中科院分区：
文献类型：
--
作者：
Cui, XF;Tao, XR;Zhou, XP
本文报道了从中国云南省不同地区的烟草、番茄和Siegesbeckia orientalis上采集的25个中国番茄黄化曲叶病毒（TYLCCNV）分离物均与DNAP分子相关。为了研究DNAP的生物学作用，将TYLCCNV分离物Y10的病毒DNA和DNAP全长感染性克隆（TYLCCNV-Y10）接种于本氏烟（Nicotianabenthamiana）、野烟（Nicotianaerososa）和烟草（Nicotiana）。tabacumSamsun（NN或nn）、番茄和矮牵牛植物。我们发现TYLCCNV-Y10单独可以全身感染这些植物，但没有诱导症状。除了TYLCCNV-Y10外，TYLCCNV-Y10 DNAP是诱导这些宿主中的曲叶病所必需的。与TYLCCNV-Y10相似，TYLCCNV分离物Y 64的DNAP也被发现是在所测试的寄主中诱导典型曲叶病所必需的。当TYLCCNV-Y10 DNAP的betaC 1基因突变时，突变体不能诱导烟草的卷叶症状。当与TYLCCNV-Y10共接种时，本塞姆氏菌。然而，Southern印迹杂交分析表明，突变的DNAP分子被复制。当N. benthamiana和N.用含有在花椰菜花叶病毒35 S启动子控制下的β C1基因的构建体转化烟草植物，许多转基因植物产生了与病毒引起的症状相似的叶片卷曲症状，其严重程度超过了β C1转录物的水平，而用在起始密码子后含有终止密码子的β C1基因转化的转基因植物保持无卷曲。因此，在没有病毒的情况下，β C1基因的表达足以诱导病毒感染的症状。
We report here that all 25 isolates of Tomato yellow leaf curl China virus (TYLCCNV) collected from tobacco, tomato, or Siegesbeckia orientalis plants in different regions of Yunnan Province, China, were associated with DNAP molecules. To investigate the biological role of DNAP, full-length infectious clones of viral DNA and DNAP of TYLCCNV isolate Y10 (TYLCCNV-Y10) were agroinoculated into Nicotiana benthamiana, Nicotiana glutinosa, Nicotiana. tabacum Samsun (NN or nn), tomato, and petunia plants. We found that TYLCCNV-Y10 alone could systemically infect these plants, but no symptoms were induced. TYLCCNV-Y10 DNAP was required, in addition to TYLCCNV-Y10, for induction of leaf curl disease in these hosts. Similar to TYLCCNV-Y10, DNAP of TYLCCNV isolate Y64 was also found to be required for induction of typical leaf curl diseases in the hosts tested. When the betaC1 gene of TYLCCNV-Y10 DNAP was mutated, the mutants failed to induce leaf curl symptoms in N. benthamiana when coinoculated with TYLCCNV-Y10. However, Southern blot hybridization analyses showed that the mutated DNAP molecules were replicated. When N. benthamiana and N. tabacum plants were transformed with a construct containing the betaC1 gene under the control of the Cauliflower mosaic virus 35S promoter, many transgenic plants developed leaf curl symptoms similar to those caused by a virus, the severity of which paralleled the level of betaC1 transcripts, while transgenic plants transformed with the betaC1 gene containing a stop codon after the start codon remained symptomless. Thus, expression of a betaC1 gene is adequate for induction of symptoms of viral infection in the absence of virus.