Decreased keratinocyte motility in skin wound on mice lacking the epidermal fatty acid binding protein gene
Decreased keratinocyte motility in skin wound on mice lacking the epidermal fatty acid binding protein gene
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DOI：
10.1007/s11010-005-9048-8
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发表时间：
2006-01
影响因子：
4.3
通讯作者：
Y. Kusakari;E. Ogawa;Y. Owada;Noriko Kitanaka;Hiroshi Watanabe;M. Kimura;H. Tagami;H. Kondo;S. Aiba;R. Okuyama
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文献类型：
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作者：
Y. Kusakari;E. Ogawa;Y. Owada;Noriko Kitanaka;Hiroshi Watanabe;M. Kimura;H. Tagami;H. Kondo;S. Aiba;R. Okuyama
脂肪酸在多种皮肤功能中发挥着重要作用。脂肪酸结合蛋白（FABP）被认为充当脂质穿梭者，溶解疏水性脂肪酸并将其输送到适当的代谢系统。在 FABP 家族蛋白中，表皮型 FABP (E-FABP) 仅在角质形成细胞中表达，但其在皮肤中的具体作用尚未完全确定。我们发现愈合伤口的再生角质形成细胞中 E-FABP 的表达升高。然而，在体内伤口闭合过程中，E-FABP缺失小鼠与野生型小鼠相比没有表现出明显差异。另一方面，在角质形成细胞培养物中，E-FABP基因破坏降低了细胞运动性，但不影响细胞增殖。 E-FABP缺失可以通过伤口周围的各种细胞（例如成纤维细胞和内皮细胞）组成的微环境在体内得到补偿。我们的分析表明，E-FABP 升高可能是伤口愈合过程中再生表皮内细胞运动激活所必需的。
Fatty acids are shown to be important in various skin functions. Fatty acid binding protein (FABP) is postulated to serve as a lipid shuttle, solubilizing hydrophobic fatty acids and delivering them to the appropriate metabolic system. Among the FABP family proteins, epidermal-type FABP (E-FABP) is solely expressed in keratinocyte but its specific role in skin is not yet fully established. We found an elevated expression of E-FABP in regenerative keratinocytes of healing wounds. However, E-FABP null mice showed no marked differences compared to wild type mice in the process of wound closure,in vivo. On the other hand, in keratinocyte culture, E-FABP gene disruption decreased the cell motility, but did not affect the cell proliferation. E-FABP deletion may be compensated forin vivoby the microenvironment comprised of various cells such as fibroblasts and endothelial cells around the wound. Our analyses suggest that the E-FABP elevation may be necessary for the activation of cell motility within regenerative epidermis during wound healing.