PROTEOLYTIC PROCESSING AND PHYSIOLOGICAL REGULATION
PROTEOLYTIC PROCESSING AND PHYSIOLOGICAL REGULATION
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DOI：
10.1016/0968-0004(89)90061-3
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发表时间：
1989-07-01
影响因子：
13.8
通讯作者：
NEURATH, H
中科院分区：
文献类型：
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作者：
NEURATH, H
蛋白质水解是一种常见而有效的生理调节机制。其基本原理是通过翻译后蛋白质水解裂解特定肽键而引起的蛋白质前体构象变化。利用蛋白质化学和分子生物学的方法，将模型酵素的早期研究扩展到更复杂的生理调节系统，极大地扩展了蛋白质水解加工反应的知识，并对当前蛋白质结构、结构域组织和进化的研究做出了重大贡献。蛋白质水解过程是多种蛋白质生物合成的最后一步。加工发生在许多不同的方式，并直接在目标蛋白的前体的特定肽键的切割。这种有限的蛋白水解取决于加工酶的特异性和可剪切肽键对攻击蛋白酶的可及性。蛋白质水解过程所调节的生理过程种类繁多（见表1）。在这篇综述中，重点关注了这一研究领域的最新进展，特别强调了代表性系统的分子机制和生理后果。
Proteolytic processing is a common and effective mechanism of physiological regulation. The basic principle is a conformational change induced in the protein precursor by the post-translational proteolytic cleavage of a specific peptide bond. The extension of earlier studies of model zymogens to more complex systems of physiological regulation, using methods of both protein chemistry and molecular biology, has enormously extended knowledge of the repertoire of proteolytic processing reactions and has contributed significantly to current studies of the structure, domain organization and evolution of proteins.Proteolytic processing is the final step in the biosynthesis of a great variety of proteins. Processing occurs in many different ways and is directed to the cleavage of specific peptide bonds in the precursor of the target protein. Such limitedproteolysis depends on the specificity of the processing enzyme and on the accessibility of the scissile peptide bonds to the attacking protease 1. The repertoire of physiological processes regulated by proteolytic processing is large and varied 2 (see Table I for examples). In this overview, attention is focused on recent developments in this field of research, with special emphasis on the molecular mechanisms and physiological consequences in representative systems.