Design, 3D-printing, and characterisation of a low-cost, open-source centrifuge adaptor for separating large volume clinical blood samples
Design, 3D-printing, and characterisation of a low-cost, open-source centrifuge adaptor for separating large volume clinical blood samples
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用于分离大容量临床血液样本的低成本开源离心机适配器的设计、3D 打印和表征
DOI：
10.1101/2021.10.19.464959
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Haque M
中科院分区：
文献类型：
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作者：
Haque M
血浆分离是许多涉及低丰度血浆生物标记物的生物医学分析的先决条件，因此是许多生物分析步骤之前的基本步骤。传统上，血浆分离是使用大容量冷冻离心机进行的，这种离心机具有处理大量血液样本的优势。这些离心机体积庞大，对于资源匮乏的环境来说，价格高得令人望而却步，起价为1500美元。尽管商用和现有的开源微型离心机成本相对较低，但它们无法处理大量血液样本。微流控血浆分离也被许多研究人员采用，以实现低成本的血浆分离，然而，这些系统仍然存在产量和纯度问题，用于极低丰度的生物标志物检测，如检测循环中不同部分的无细胞DNA。为了克服这一问题，我们定制了一台商用微型离心机的转子，采用熔丝制造，使其能够在资源匮乏的环境中对大量临床血液样本进行离心分离。我们设计的适配器(15美元)可以容纳两个9毫升的S-莫诺瓦特管，并保持与控制台式冷冻离心机相同的分离性能(产量、细胞计数、溶血和白蛋白水平)。这种能够处理临床血液管的低成本开源离心机系统对于资金不足的实验室或需要在抽血后立即进行进一步检测的低资源环境来说可能很有价值。
Blood plasma separation is a prerequisite in numerous biomedical assays involving low abundance plasma-borne biomarkers and thus is the fundamental step before many bioanalytical steps. Conventionally, plasma separation is performed using high-capacity refrigerated centrifuges which have the advantage of handling large volume blood samples. These centrifuges are bulky, and prohibitively expensive for low-resource settings, with prices starting from $1,500. Although commercial and existing open-source micro-centrifuges are relatively low-cost, they cannot handle large volume blood samples. Microfluidic blood plasma separation also has been adopted by many researchers to enable low-cost plasma separation, however, these systems still present yield and purity issues for extremely low abundance biomarker detection such as the detection of various fractions of circulating cell-free DNA. To overcome this, we customised the rotor of a commercially available micro-centrifuge ∼$125) using fused filament fabrication to enable centrifugation of large clinical blood samples in resource poor-settings. Our designed adaptor ($15) can hold two 9 mL S-Monovette tubes and maintain the same separation performance (yield, cell count, hemolysis, albumin levels) as the control benchtop refrigerated centrifuge. This low-cost open-source centrifugation system capable of processing clinical blood tubes could be valuable to low-funded laboratories or low-resource settings where centrifugation is required immediately after blood withdrawal for further testing.