Progressive cis-inhibition of telomerase upon telomere elongation
Progressive cis-inhibition of telomerase upon telomere elongation
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DOI：
10.1093/emboj/18.12.3509
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发表时间：
1999-06-15
期刊：
影响因子：
11.4
通讯作者：
Gilson, E
中科院分区：
文献类型：
--
作者：
Marcand, S;Brevet, V;Gilson, E
在酵母中，端粒DNA的恒定长度是由于端粒本身对端粒酶的负调节。在这里，我们遵循异常缩短的端粒恢复平衡，我们观察到端粒延长被限制在每代几个碱基对，并且其速率随着端粒长度的增加而逐渐降低，相反，在不存在端粒酶或存在过度延长的端粒的情况下，与世代连续相关的降解速率似乎是恒定的，即不依赖于端粒长度。总之，这些结果表明端粒酶在其作用位点被延长的端粒逐渐抑制。在这项研究中，这一发现的端粒长度调节的动力学的影响进行了讨论。
In yeast, the constant length of telomeric DNA results from a negative regulation of telomerase by the telomere itself. Here we follow the return to equilibrium of an abnormally shortened telomere, We observe that telomere elongation is restricted to a few base pairs per generation and that its rate decreases progressively with increasing telomere length, In contrast, in the absence of telomerase or in the presence of an over-elongated telomere, the degradation rate linked to the succession of generations appears to be constant, i.e. independent of telomere length, Together, these results indicate that telomerase is gradually inhibited at its site of action by the elongating telomere. The implications of this finding for the dynamics of telomere length regulation are discussed in this study.