Isolation and characterization of dermatan sulphate proteoglycan from human uterine cervix.
Isolation and characterization of dermatan sulphate proteoglycan from human uterine cervix.
复制标题
从人子宫颈中分离和表征硫酸皮肤素蛋白多糖。
作者：
N. Uldbjerg;A. Malmström;G. Ekman;J. Sheehan;U. Ulmsten;L. Wingerup
在蛋白酶抑制剂存在下，用4 M-氯化胍从人宫颈中提取蛋白多糖。它们通过在4 M-氯化胍/CsCl（起始密度1.32 g/ml）中密度梯度离心，随后通过DEAE-纤维素和琼脂糖色谱法纯化。结果表明，该蛋白多糖只有一种多分散性，s 020，w = 2.1S，重均分子量为73000（沉降-平衡离心法）~ 110500（光散射法）。核心蛋白是单分散的，具有47 000的表观分子量。蛋白多糖含有约30%的蛋白质，每个分子可能含有两个或三个糖胺聚糖侧链。天冬氨酸，谷氨酸和亮氨酸的含量高，被发现。的聚糖部分的蛋白聚糖是专门的硫酸皮肤素，与大约等量的艾杜糖醛酸和葡萄糖醛酸含有二糖的共聚物结构。
Proteoglycans were extracted from human uterine cervix with 4 M-guanidinium chloride in the presence of proteinase inhibitors. They were purified by density-gradient centrifugation in 4 M-guanidinium chloride/CsCl (starting density 1.32 g/ml) followed by DEAE-cellulose and Sepharose chromatography. Only one polydisperse proteoglycan was found. s020,w was 2.1S and the weight-average molecular weight was 73 000 (sedimentation-equilibrium centrifugation) to 110 500 (light-scattering). The core protein was monodisperse, with an apparent molecular weight of 47 000. The proteoglycan contained about 30% protein and probably two or three glycosaminoglycan side chains per molecule. High contents of aspartate, glutamate and leucine were found. The glycan moiety of the proteoglycan was exclusively dermatan sulphate, with a co-polymeric structure with approximately equal quantities of iduronic acid- and glucuronic acid-containing disaccharides.