The multiplicity of Plasmodium falciparum infections is associated with acquired immunity to asexual blood stage antigens
The multiplicity of Plasmodium falciparum infections is associated with acquired immunity to asexual blood stage antigens
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DOI：
10.1016/j.micinf.2008.10.012
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发表时间：
2009-01-01
影响因子：
5.8
通讯作者：
Ntoumi, Francine
中科院分区：
文献类型：
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作者：
Mayengue, Pembe Issarnou;Luty, Adrian J. F.;Ntoumi, Francine
我们评估了免疫反应标志物与恶性疟原虫感染的多重性之间的关系，以评估后者作为获得抗疟疾免疫力的指标的有效性。使用基于 MSP-1 和 MSP-2 PCR 的方法对 64 名加蓬儿童的疟疾发作期间存在的寄生虫种群进行了表征，这些儿童在 7 年的积极随访期间至少出现过 4 次此类攻击。在健康和无寄生虫阶段采集的血浆样本用于测量恶性疟原虫抗原特异性抗体和细胞因子活性。我们发现不同疟疾发作中存在的寄生虫基因型数量存在个体内和个体间差异的证据，尽管在72%的分离株中检测到不超过2种寄生虫基因型。多重性最高的样本来自对特定无性阶段抗原的抗体反应显着较低（p < 0.03）的儿童。此外，感染复数较低的人的全血干扰素-γ产生能力显着较高（p < 0.02）。具有多个克隆的疟疾发作确实反映了获得性免疫水平较低，因此控制感染的能力较差。这些发现表明，恶性疟原虫感染的多重性可能是评估疟疾控制干预措施的潜在有用参数。 (c) 2008 年 Elsevier Masson SAS。版权所有。
We evaluated the relationship between immune response markers and the multiplicity of Plasmodium falciparum infections in order to assess the validity of the latter as an indicator of the acquisition of anti-malarial immunity.Parasite populations present during malaria episodes of 64 Gabonese children who presented with at least 4 such attacks during active follow-up over a 7-year period were characterized using MSP-1 and MSP-2 PCR-based methods. Plasma samples taken at healthy and parasite-free phase were used to measure P. falciparum antigen-specific antibody and cytokine activity.We found evidence of intra- and inter-individual variation in the number of parasite genotypes present in different malaria episodes, although in 72% of isolates no more than 2 parasite genotypes were detectable. Samples with the highest multiplicity were from children with significantly lower (p < 0.03) antibody responses to specific asexual stage antigens. Additionally, the whole blood interferon-gamma production capacity was significantly higher (p < 0.02) in those with lower infection multiplicity.Malaria episodes with multiple clones indeed reflect a low level of acquired immunity and a consequently poor capacity to control the infection. These findings suggest that the multiplicity of falciparum infection may be a potentially useful parameter in the evaluation of malaria control interventions. (c) 2008 Elsevier Masson SAS. All rights reserved.