Expression of leptin receptors and induction of IL-1β transcript in glial cells
Expression of leptin receptors and induction of IL-1β transcript in glial cells
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DOI：
10.1006/bbrc.2000.2937
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发表时间：
2000-06-24
影响因子：
3.1
通讯作者：
Nomura, Y
中科院分区：
文献类型：
--
作者：
Hosoi, T;Okuma, Y;Nomura, Y
为了研究瘦素在大脑免疫功能中的作用，我们检测了瘦素对小鼠原代培养的胶质细胞中白细胞介素-1 β（IL-1 β）表达的影响。RT-PCR检测原代培养的胶质细胞中瘦素受体亚型Ob-Ra和Ob-Rb mRNA的表达。Western blotting分析显示原代培养的胶质细胞也表达瘦素受体蛋白。用RT-PCR方法检测瘦素对IL-1 β mRNA表达的影响。IL-1 β转录本的表达在应用瘦素后2 ~ 6 h达到高峰。这些结果表明，瘦素可以诱导IL-1 β在脑中的转录，并且瘦素诱导的IL-1 β转录的靶细胞之一可能是神经胶质细胞。(C)北京大学出版社.
To examine the role of leptin in the immune function of the brain, we examined the effect of leptin on interleukin-1 beta (IL-1 beta) expression in mouse primary cultured glial cells. The expression of leptin receptor isoforms Ob-Ra and Ob-Rb mRNA was detected by RT-PCR analysis of total RNA from primary cultured glial cells. Protein of leptin receptor was also expressed in mouse primary cultured glial cells as evaluated by Western blotting analysis. Leptin increased the expression of IL-1 beta mRNA evaluated by RT-PCR. The expression of IL-1 beta transcript peaked 2 to 6 h after leptin application. These results indicate that leptin could induce IL-1 beta transcript in the brain and that one of the target cells of the leptin-induced IL-1 beta transcript may be a glial cell. (C) 2000 Academic Press.