Magnetic Dynabeads® detection by sensitive element based on giant magnetoimpedance
Magnetic Dynabeads® detection by sensitive element based on giant magnetoimpedance
复制标题
DOI：
10.1016/j.bios.2004.04.027
复制
发表时间：
2005-02-15
影响因子：
12.6
通讯作者：
Levit, V
中科院分区：
文献类型：
--
作者：
Kurlyandskaya, G;Levit, V
设计并制作了一种基于非晶带的转基因生物传感器样机。作为第一步，用模型液体介质测量GMI响应：含有可磁化Dynabeads（R）M-450的悬浮液。当用特异性抗体包被时，这些珠子可以用作生物分子标记。由它们的存在引起的巨磁阻抗变化测量与生物传感器原型设计的测量单元含有非晶CoFeMoSiB-带状元素。巨磁阻抗是在0.3至10 MHz的电流频率范围内和在I-rms = 3或4.5 mA的强度下测量的。商业Dynabeads（R）M-450以磷酸盐缓冲盐水中的悬浮液形式提供。在3.5 MHz和1 = 3 mA的频率下，在使用和不使用Dynabeads（R）的情况下测量的GMI比的最大差异为25%。最后提出了转基因生物传感器的应用前景和进一步研究的方向。(C)2004 Elsevier B. V.保留所有权利。
A GMI-biosensor prototype was designed and fabricated using an amorphous ribbon. As a first step, a GMI response was measured with a model liquid media: suspension containing magnetisable Dynabeads(R) M-450. These beads can be used as biomolecular labels when coated with a specific antibody. The GMI change caused by their presence was measured with a biosensor prototype designed with a measuring cell containing an amorphous CoFeMoSiB-ribbon element. GMI was measured at a range of current frequencies from 0.3 to 10 MHz and at intensities of either I-rms = 3 or 4.5 mA. Commercial Dynabeads(R) M-450 were supplied as a suspension in a phosphate buffered saline. A maximum difference of 25% in the GMI ratio measured with and without Dynabeads(R) was obtained at a frequency of 3.5 MHz and 1 = 3 mA. Some potential applications for GMI-biosensor and further directions are proposed. (C) 2004 Elsevier B.V. All rights reserved.