Selective induction of mitogen-activated protein kinases in human lens epithelial cells by ultraviolet radiation
Selective induction of mitogen-activated protein kinases in human lens epithelial cells by ultraviolet radiation
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DOI：
10.1002/jbt.10016
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发表时间：
2002-01-01
影响因子：
3.6
通讯作者：
Bomser, JA
中科院分区：
文献类型：
--
作者：
Bomser, JA
本研究探讨了紫外线辐射（UVR）对人晶状体上皮（HLE）细胞中丝裂原活化蛋白激酶（MAPK）活性的影响。紫外线B和紫外线C照射HLE细胞激活应激反应MAPK蛋白p38和C - jun nh2末端激酶（JNK），并以剂量和时间依赖的方式，而细胞外调节信号激酶（ERK 44/42）级联不受紫外线照射的影响。紫外线A辐射未能引起MAPK响应。uvr诱导的MAPK激活不需要蛋白激酶C或磷脂酰肌醇3激酶活性，这表明这不是一个受体介导的事件。核糖体翻译的抑制完全消除了uvr诱导的MAPK激活，而抗氧化剂、n -乙酰半胱氨酸和轻度热休克对这种激活没有影响。这些数据首次证明了MAPK在晶状体上皮细胞系中的选择性级联激活。(C) 2002 Wiley期刊有限公司
The present study investigates the effects of ultraviolet radiation (UVR) on mitogen-activated protein kinase (MAPK) activity in human lens epithelial (HLE) cells. Irradiation of HLE cells with ultraviolet B and ultraviolet C radiation activates the stress-response MAPK proteins, p38 and c-Jun NH2-terminal kinase (JNK), in a dose- and time-dependent manner, while the extracellular-regulated signal kinase (ERK 44/42) cascade was not altered by UVR exposure. Ultraviolet A radiation failed to elicit a MAPK response. UVR-induced MAPK activation does not require protein kinase C or phosphatidylinositol 3-kinase activity, suggesting that this is not a receptor-mediated event. Inhibition of ribosomal translation completely abolished UVR-induced MAPK activation, while treatment with the antioxidant, N-acetyl cysteine, and mild heat shock had no effect on this activation. These data demonstrate for the first time the selective activation of MAPK cascades in a lens epithelial cell line. (C) 2002 Wiley Periodicals, Inc.