YEAST MUTANTS DEFICIENT IN PROTEIN GLYCOSYLATION
YEAST MUTANTS DEFICIENT IN PROTEIN GLYCOSYLATION
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DOI：
10.1073/pnas.80.24.7466
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
ROBBINS, PW
中科院分区：
文献类型：
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作者：
HUFFAKER, TC;ROBBINS, PW
天冬酰胺连接的寡糖的合成涉及具有组成Glc 3 Man 9 GlcNAc 2的脂质连接的前体寡糖的形成。[3 H]甘露糖自杀选择用于在酵母中获得在该前体寡糖的合成中被阻断的突变体。alg 1突变体积累脂质连接的GlcNAc 2，alg 2突变体积累Man 1 - 2GlcNAc 2，alg 3突变体积累Man 5GlcNAc 2，alg 4突变体积累Man 1 - 8 GlcNAc 2，alg 5和alg 6突变体积累Man 9 GlcNAc 2。这些突变体中的一些似乎将除Glc 3-Man 9 GlcNAc 2之外的寡糖从脂质载体转移到转化酶。这些异常的蛋白质连接的低聚糖加工的alg 3，alg 5和alg 6突变体的外链残基。在alg 2和alg 4突变体中几乎没有外链添加。alg 4突变体是唯一不能分泌转化酶的突变体。
The synthesis of asparagine-linked oligosaccharides involves the formation of a lipid-linked precursor oligosaccharide that has the composition Glc3Man9GlcNAc2. [3H]mannose suicide selection was used to obtain mutants in yeast that are blocked in the synthesis of this precursor oligosaccharide. The alg1 mutant accumulated lipid-linked GlcNAc2, alg2 mutants accumulated Man1-2GlcNAc2, alg3 mutants accumulated Man5GlcNAc2, alg4 mutants accumulated Man1-8GlcNAc2 and alg5 and alg6 mutants accumulated Man9GlcNAc2. Some of these mutants appeared to transfer oligosaccharides other than Glc3-Man9GlcNAc2 from the lipid carrier to invertase. These aberrant protein-linked oligosaccharides were processed by the addition of outer chain residues in the alg3, alg5 and alg6 mutants. There was virtually no outer chain addition in the alg2 and alg4 mutants. The alg4 mutant was the only mutant that failed to secrete invertase.