Characterization of an alkylated dinucleotide by desorption chemical ionization and tandem mass spectrometry.
Characterization of an alkylated dinucleotide by desorption chemical ionization and tandem mass spectrometry.
复制标题
通过解吸化学电离和串联质谱法表征烷基化二核苷酸。
DOI：
10.1002/bms.1200140106
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Chang,CJ
中科院分区：
文献类型：
--
作者：
Isern-Flecha,I;Jiang,XY;Cooks,RG;Pfleiderer,W;Chae,WG;Chang,CJ
开发了一种高灵敏度（亚皮摩尔水平）、结构特异性分析方法，用于通过解吸化学电离串联质谱法表征和定量二核苷酸胸苷酰（3′ → 5′）胸苷甲基磷酸三酯。该方法可应用于相关化合物，如母体二核苷酸，并采用正或负电离质谱或子谱。该程序允许甲基连接位点的规格。
A highly sensitive (subpicomole level), structure‐specific method of analysis has been developed for characterizing and quantitating the dinucleotide thymidylyl(3′ → 5′)thymidine methyl phosphotriester by desorption chemical ionization tandem mass spectrometry. The methodology can be applied to related compounds such as the parent dinucleotide and employs either positive or negative ionization mass spectra or daughter spectra. The procedure allows specification of the site of methyl attachment.