Mesoscale imaging with cryo-light and X-rays: Larger than molecular machines, smaller than a cell.
Mesoscale imaging with cryo-light and X-rays: Larger than molecular machines, smaller than a cell.
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具有冷冻和X射线的中尺度成像：比分子机大，比单元小。
DOI：
10.1111/boc.201600044
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发表时间：
2017-01
影响因子：
2.7
通讯作者：
Larabell CA
中科院分区：
文献类型：
--
作者：
Ekman AA;Chen JH;Guo J;McDermott G;Le Gros MA;Larabell CA
在细胞生物学的背景下，术语中尺度描述了从单个细胞的长度到分子机器的大小的长度尺度。在这种空间体系中，小的积木自组织起来，形成大型的功能结构。一套全面的中尺度自组织规则尚未建立，这使得对许多细胞行为的预测变得困难，如果不是不可能的话。我们对中尺度生物学的知识来自实验数据，特别是成像。在这里，我们探索了软X射线层析成像(SXT)在中尺度成像中的应用，并描述了这项技术可以产生的结构洞察。我们还讨论了如何通过添加从同一细胞测量的相关荧光数据来补充SXT成像。这两种离散成像模式的组合产生了细胞的3D视图，将高分辨率结构信息与精确的分子定位数据相结合。
In the context of cell biology, the term mesoscale describes length scales ranging from that of an individual cell, down to the size of the molecular machines. In this spatial regime, small building blocks self-organise to form large, functional structures. A comprehensive set of rules governing mesoscale self-organisation has not been established, making the prediction of many cell behaviours difficult, if not impossible. Our knowledge of mesoscale biology comes from experimental data, in particular, imaging. Here, we explore the application of soft X-ray tomography (SXT) to imaging the mesoscale, and describe the structural insights this technology can generate. We also discuss how SXT imaging is complemented by the addition of correlative fluorescence data measured from the same cell. This combination of two discrete imaging modalities produces a 3D view of the cell that blends high-resolution structural information with precise molecular localisation data.