Significance of cell-to cell contacts for the directional movement of neural crest cells within a hydrated collagen lattice.
Significance of cell-to cell contacts for the directional movement of neural crest cells within a hydrated collagen lattice.
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发表时间：
1981-06
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通讯作者：
E. M. Davis;J. Trinkaus
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作者：
E. M. Davis;J. Trinkaus
神经嵴刚刚开始迁移的神经管从阶段14鸡胚中分离，用0.1%胰蛋白酶清洗，并在透明水合胶原晶格（HCL）中培养，以部分刺激胚胎中神经嵴细胞原位迁移的三维环境。晶格中胶原蛋白的浓度从50微克/毫升到390微克/毫升不等。在这些条件下从神经管迁移的神经嵴细胞的运动和接触行为的模式直接记录与延时电影显微摄影。它们的形状和它们的移位率都依赖于HCL中胶原的浓度。以低浓度（50微克/毫升至105微克/毫升），神经嵴细胞具有细长的梭形形状，并以1微米/分钟的平均速率移位，而在高浓度下，（190微克/毫升至390微克/毫升），其形状为圆形，并且它们以仅0-5微米/分钟的平均速率移位。在这些制剂中神经嵴细胞主要以松散的簇从神经管迁移，几个单细胞处于领先地位这些组中的细胞显示出前缘到后缘的粘附，并形成远离神经管的细胞舌或细胞流。单个细胞和细胞群的迁移路径与HCL的胶原原纤维对齐，其从神经管辐射。没有观察到运动的接触抑制的经典可见特征，即与其他细胞接触后细胞运动方向的变化;易位率和迁移离开管所花费的时间都不依赖于细胞之间的接触次数。结论是，在HCL培养的神经嵴细胞的定向运动不依赖于运动的接触抑制。
Neural tubes whose neural crest had just begun migration were isolated from stage-14 chick embryos, cleaned with 0.1% trypsin, and cultured in transparent hydrated collagen lattices (HCL) in an effect to stimulate in part the three-dimensional environment through which neural crest cells migrate in situ, in the embryo. The concentration of collagen in the lattices varied from 50 microgram/ml to 390 microgram/ml. The mode of movement and contact behaviour of neural crest cells migrating from the neural tube under these conditions were recorded directly with time-lapse cinemicrography. Both their shape and their rate of translocation were dependent on the concentration of collagen in the HCL. In low concentrations (50 microgram/ml to 105 microgram/ml), neural crest cells have elongate spindle shapes and translocate at an average rate of 1 micrometer/min, whereas in high concentrations (190 microgram/ml to 390 microgram/ml), their shape is rounded, and they translocate at an average rate of only 0-5 micrometer/min. Neural crest cells migrate from neural tubes in these preparations principally in loose clusters, with a few single cells in the lead. The cells in these groups display leading-to-trailing edge adhesions and form tongues or streams of cells directed away from the neural tube. The paths of migration of both individual cells and groups of cells are aligned with the collagen fibrils of the HCL, which radiate from the neural tube. The classical visible characteristic of contact inhibition of movement, change in direction of cell movement after contact with other cells, was not observed; neither the rate of translocation nor the time spent migrating away from the tube is dependent on the number of contacts between cells. It is concluded that the directional movement of neural crest cells in HCL cultures does not depend on contact inhibition of movement.