Leaf hydraulic conductance varies with vein anatomy across Arabidopsis thaliana wild-type and leaf vein mutants
Leaf hydraulic conductance varies with vein anatomy across Arabidopsis thaliana wild-type and leaf vein mutants
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DOI：
10.1111/pce.12584
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发表时间：
2015-12-01
影响因子：
7.3
通讯作者：
Sack, Lawren
中科院分区：
文献类型：
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作者：
Caringella, Marissa A.;Bongers, Franca J.;Sack, Lawren
不同植物物种的叶脉是多种多样的，并且具有从古植物学到现代农业的实际应用。然而，静脉性状对植物性能的影响尚未在拟南芥等模型系统中进行测试。先前的研究分析了拟南芥静脉突变体的子叶，并确定了其静脉系统与野生型（WT）的明显差异。我们测量了拟南芥WT (Col-0) 和四种叶脉突变体（dot3-111 和 dot3-134、cvp1-3 和 cvp2-1）的叶水力导度（K-leaf）、叶脉特征以及木质部和叶肉解剖结构。突变的真叶不具有先前在子叶中显示的定性脉络异常，但在不同基因型的脉特征和叶解剖结构上存在数量上的变化。 WT 的平均 K 叶明显更高。在所有基因型中，K 叶与束鞘水力传导相关的性状有很强的相关性，受束鞘细胞数量和径向直径以及单位面积静脉长度的影响。这些发现支持了静脉特征影响 K 叶的假设，表明该突变系统对于测试主要在物种间比较建立的理论的有用性，并支持束鞘在影响 K 叶中的强大作用。
Leaf venation is diverse across plant species and has practical applications from paleobotany to modern agriculture. However, the impact of vein traits on plant performance has not yet been tested in a model system such as Arabidopsis thaliana. Previous studies analysed cotyledons of A.thaliana vein mutants and identified visible differences in their vein systems from the wild type (WT). We measured leaf hydraulic conductance (K-leaf), vein traits, and xylem and mesophyll anatomy for A.thalianaWT (Col-0) and four vein mutants (dot3-111 and dot3-134, and cvp1-3 and cvp2-1). Mutant true leaves did not possess the qualitative venation anomalies previously shown in the cotyledons, but varied quantitatively in vein traits and leaf anatomy across genotypes. The WT had significantly higher mean K-leaf. Across all genotypes, there was a strong correlation of K-leaf with traits related to hydraulic conductance across the bundle sheath, as influenced by the number and radial diameter of bundle sheath cells and vein length per area. These findings support the hypothesis that vein traits influence K-leaf, indicating the usefulness of this mutant system for testing theory that was primarily established comparatively across species, and supports a strong role for the bundle sheath in influencing K-leaf.