Plastic modifications induced by object recognition memory processing
Plastic modifications induced by object recognition memory processing
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DOI：
10.1073/pnas.0915059107
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发表时间：
2010-02-09
影响因子：
11.1
通讯作者：
Delgado-Garcia, Jose Maria
中科院分区：
文献类型：
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作者：
Clarke, Julia Rosauro;Cammarota, Martin;Delgado-Garcia, Jose Maria
长时程增强（LTP）现象被广泛认为是记忆巩固的细胞模型。物体识别（OR）是研究啮齿动物陈述性记忆的一种特别有用的方法，因为它利用了啮齿动物对新奇物体的先天偏好。在这项研究中，小鼠在海马Schaffer侧支-锥体CA 1通路中植入电极，并接受OR训练。在训练时和训练后不同时间记录CA 3-CA 1突触诱发的EPSP。LTP样突触增强在训练后6 h出现。训练后24小时进行测试，在一个熟悉的和一个新的对象的存在。海马突触易化过程中观察到的熟悉和新的对象的探索。在测试后早期观察到短的去增强期，随后是突触功效增强的后期。在这里，我们表明，或记忆巩固伴随着短暂的增强海马CA 3-CA 1突触，而这种记忆的重新巩固需要一个短暂的去增强阶段，可以解释其良好的描述脆弱性。另一方面，后期突触增强期可能是记忆重新稳定的结果。
Long-term potentiation (LTP) phenomenon is widely accepted as a cellular model of memory consolidation. Object recognition (OR) is a particularly useful way of studying declarative memory in rodents because it makes use of their innate preference for novel over familiar objects. In this study, mice had electrodes implanted in the hippocampal Schaffer collaterals-pyramidal CA1 pathway and were trained for OR. Field EPSPs evoked at the CA3-CA1 synapse were recorded at the moment of training and at different times thereafter. LTP-like synaptic enhancement was found 6 h posttraining. A testing session was conducted 24 h after training, in the presence of one familiar and one novel object. Hippocampal synaptic facilitation was observed during exploration of familiar and novel objects. A short depotentiation period was observed early after the test and was followed by a later phase of synaptic efficacy enhancement. Here, we show that OR memory consolidation is accompanied by transient potentiation in the hippocampal CA3-CA1 synapses, while reconsolidation of this memory requires a short-lasting phase of depotentiation that could account for its well described vulnerability. The late synaptic enhancement phase, on the other hand, would be a consequence of memory restabilization.