An autonomous drug release system based on chemo-mechanical energy conversion "Organic Engine" for feedback control of blood glucose.
An autonomous drug release system based on chemo-mechanical energy conversion "Organic Engine" for feedback control of blood glucose.
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DOI：
10.1016/j.bios.2010.07.080
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发表时间：
2010-12
影响因子：
12.6
通讯作者：
Ryodai Kato;Munkhbayar Munkhjargal;D. Takahashi;T. Arakawa;H. Kudo;K. Mitsubayashi
中科院分区：
文献类型：
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作者：
Ryodai Kato;Munkhbayar Munkhjargal;D. Takahashi;T. Arakawa;H. Kudo;K. Mitsubayashi
制备并测试了一种新型自主药物释放系统。该系统由两个集成单元组成：减压单元和药物释放单元。减压单元由葡萄糖氧化酶（GOD）酶固定膜将圆柱形细胞分为顶部细胞（气相）和底部细胞（液相）制成。酶膜识别葡萄糖并将葡萄糖中的化学能转化为机械能。当底部电池施加葡萄糖溶液时，顶部电池的降压斜率与葡萄糖浓度呈线性相关。之后，利用减压单元的能量作为动力源的药物释放单元被制造并通过记录其释放行为来评价。使药物释放单元在液相中以恒定量释放药物。然后通过组合减压单元和药物释放单元来制造系统。并通过应用葡萄糖溶液（100 mmol/l）和NADH+的混合物，使用葡萄糖脱氢酶（GDH）作为葡萄糖还原剂，在开环和闭环中进行评价。葡萄糖浓度在闭环中逐渐降低，因此，GDH释放的间隔时间变长。换句话说，示出了系统的致动间隔与葡萄糖浓度之间的逆相关性。结果，证实了在没有外部能量的情况下通过药物释放系统反馈控制葡萄糖浓度的可能性。
A novel autonomous drug release system was fabricated and tested. The system consists of two integrated units: decompression unit and drug release unit. The decompression unit was fabricated by separating a cylindrical cell into a top cell (gas phase) and a bottom cell (liquid phase) by glucose oxidase (GOD) enzyme immobilized membrane. The enzyme membrane recognizes glucose and converts chemical energy found in glucose to mechanical energy. The linear correlation between glucose concentration and de-pressure slope of the top cell was revealed as applying glucose solution to the bottom cell. Afterward, the drug release unit which utilizes the energy of the decompression unit as a power source was fabricated and evaluated by recording its release actions. The drug release unit was made to release at a constant quantity of drug in the liquid phase. The system was then fabricated by combining the decompression unit and the drug release unit. And it was evaluated in an open loop and in a closed loop by applying a mixture of glucose solution (100mmol/l) and NADH+using glucose dehydrogenase enzyme (GDH) as a glucose reducer. Glucose concentration decreased gradually in the closed loop and, as a consequence, interval time of the GDH release became longer. In other words, an inverse correlation between actuation interval of the system and glucose concentration was shown. As a result, the possibility of feedback control of glucose concentration by the drug release system without external energy was confirmed.