Differential induction of proteins in Anopheles gambiae sensu stricto (Diptera: Culicidae) larvae in response to heavy metal selection
Differential induction of proteins in Anopheles gambiae sensu stricto (Diptera: Culicidae) larvae in response to heavy metal selection
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DOI：
10.1017/s1742758406658955
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发表时间：
2006-12-01
影响因子：
1.2
通讯作者：
Beier, John
中科院分区：
文献类型：
--
作者：
Mireji, Paul Odhiambo;Keating, Joseph;Beier, John
进行调查，以建立由于三龄冈比亚按蚊s.s.的世代选择而导致的蛋白质差异表达的幅度和模式。黄鳝幼虫受镉、铜和铅这三种重金属的影响，可能是城市常见的污染物。A.冈比亚有限公司将三龄幼虫分别置于LC30的镉、铜和铅的选择压力下，并通过五个世代进行对照。第一，第三和第五代选择幸存者进行了筛选差异表达蛋白质相对于非暴露对照组的双向凝胶电泳。通过x（2）或Fishers精确检验分析斑点的分布模式：通过ANOVA分析代间和代内表达的变化。在所有的金属和世代中，大多数差异表达的斑点是酸性的和低分子量的。重金属种类和世代是差异表达变异的主要指标。镉选择群体的世代间变异最为显著，第5代诱导的斑点数最多。铜选择群体在第三代出现的斑点最多。诱导蛋白点可能是重金属胁迫下相应基因的产物，从而构建了A.冈比亚有限公司对这些污染物的耐受性。表达斑点的差异模式和大小具有潜在的应用价值，作为分子标记评估按蚊对重金属的适应状态，并提供洞察环境污染的程度。
Investigations were conducted to establish the magnitude and pattern of differential expression of proteins due to generational selection of third instar Anopheles gambiae s.s. Giles larvae by cadmium, copper and lead heavy metals, the three possible common urban pollutants. A susceptible strain of A. gambiae s.s. third instar larvae was separately placed under selection pressure with cadmium, copper and lead at LC30 and controls through five generations. First, third and fifth generation selection survivors were screened for differentially expressed proteins relative to non-exposed control by two-dimensional gel electrophoresis. Distribution patterns of the spots were analyzed by x(2) or Fishers' exact test: and variations in expressions between and within generations by ANOVA. Most differentially expressed spots were acidic and of low molecular weight among all metals and generations. Type of heavy metal and generation were the main indicators of variations in differential expressions. Variation between generations was most significant among cadmium-selected populations of which the most number of spots were induced in the fifth generation. Most spots were induced in the copper-selected population in the third generation. The induced protein spots may be the products from respective genes that respond to heavy metals and counter their toxicity, thus building A. gambiae s.s. tolerance to these pollutants. The differential pattern and magnitude of expressed spots have potential application as molecular markers for assessment of anopheline adaptation status to heavy metals, and provide insight into the extent of environmental pollution.