The role of 20E biosynthesis relative gene Shadow in the reproduction of the predatory mirid bug, Cyrtorhinus lividipennis (Hemiptera: Miridae)
The role of 20E biosynthesis relative gene Shadow in the reproduction of the predatory mirid bug, Cyrtorhinus lividipennis (Hemiptera: Miridae)
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20E生物合成相关基因Shadow在捕食性蝽Cyrtorhinus lividipennis（半翅目：蝽科）繁殖中的作用
DOI：
10.1002/arch.21854
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发表时间：
2021-11-15
影响因子：
2.2
通讯作者：
Liu, Fang
中科院分区：
文献类型：
--
作者：
Hu, Kui;Fu, Baobao;Liu, Fang
黑翅小蜂是稻飞虱和叶蝉的天敌。提高C.青翅夜蛾对害虫的防治效果将有所提高。然而，对C.青翅类本研究探讨了20-羟基蜕皮激素（20-hydroxyecdysone，20 E）生物合成相关基因Shadow在家蚕繁殖过程中的作用。青翅类ClSad基因全长2018 bp，开放阅读框1398 bp，编码465个氨基酸残基。ClSad在若虫和成虫期均能检测到，在成虫期高表达。ClSad在成年雌性的中肠和卵巢中高度表达。此外，RNA干扰介导的ClSad敲低降低了20 E滴度和ClVg转录物水平，导致更少的完全发育的卵和dsSad注射的成年雌性在15天内产卵的数量减少。这些结果表明，ClSad在C.青翅类本研究为深入了解ClSad基因在C.青翅类
Cytorhinus lividipennis is a natural enemy of rice planthoppers and leafhoppers. Improving the fecundity of C. lividipennis will be helpful to improve its control effect on pests. However, little is known about the hormonal regulatory mechanism of reproduction in C. lividipennis. In the current study, we examined the role of 20-hydroxyecdysone (20E) biosynthesis relative gene Shadow in the reproduction of C. lividipennis. The complementary DNA sequence of ClSad is 2018 -bp in length with an open reading frame of 1398-bp encoding 465 amino acid residues. ClSad was readily detected in nymphal and adult stages, and highly expressed in the adult stage. ClSad was highly expressed in the midgut and ovaries of adult females. Moreover, RNA interference-mediated knockdown of ClSad reduced the 20E titers and ClVg transcript level, resulting in fewer fully developed eggs and a decrease in the number of eggs laid by dsSad-injected adult females within 15 days. These results suggest that ClSad plays a critical role in the reproduction of C. lividipennis. The present study provides insights into the molecular mechanism of the ClSad gene for the reproduction of C. lividipennis.