Quantitative analysis of small molecules by desorption electrospray ionization mass spectrometry from polytetrafluoroethylene surfaces
Quantitative analysis of small molecules by desorption electrospray ionization mass spectrometry from polytetrafluoroethylene surfaces
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DOI：
10.1002/rcm.3377
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发表时间：
2008-01-01
影响因子：
2
通讯作者：
Cooks, R. Graham
中科院分区：
文献类型：
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作者：
Ifa, Demian R.;Manicke, Nicholas E.;Cooks, R. Graham
解吸电喷雾电离质谱（DES-MS）是一种新兴的环境分析技术。然而，它的应用程序，以常规定量还没有被广泛探讨，这是在这里进行。我们目前的研究，利用一个特别合适的表面，多孔聚四氟乙烯（多孔PTFE），这表明样品之间的交叉污染少，提高灵敏度和信号稳定性相比，其他表面。使用同位素标记的普萘洛尔作为内标物，对1 μ L β-受体阻滞剂普萘洛尔点样溶液进行定量实验，结果显示在0.01-100 μ M范围内具有良好的线性相关性（r（2）> 0.996）。日间精密度，相对标准偏差的基础上，重复分析在三个不同的日子是13%的0.01 μ M和优于7%的其余校准点。对于沿着曲线的所有校准点，日间准确度（表示为相对误差）优于+/-7%。这些日常的测量表明，EST-MS可以成功地用于常规定量分析。使用的类似物阿替洛尔作为内标物和进一步的考虑，应提高定量的EST-MS也提出。版权所有（C）2008约翰威利父子有限公司
Desorption electrospray ionization mass spectrometry (DESI-MS) is an emerging technique for ambient analysis. However, its application to routine quantitation has not been explored extensively and this is undertaken here. We present studies that utilize a particularly suitable surface, porous polytetrafluoroethylene (porous PTFE), which shows less cross contamination between samples and improved sensitivity and signal stability compared to other surfaces. Quantitative experiments for 1 mu L spotted solutions of the beta-blocker propranolol, using isotopically labeled propranolol as internal standard, showed a good linear correlation (r(2) > 0.996) over the range 0.01-100 mu M. The inter-day precision, based on the relative standard deviation, for replicates analyzed on three different days was 13% for 0.01 mu M and better than 7% for the remainder of the calibration points. The inter-day accuracy, expressed as relative error, was better than +/-7% for all calibration points along the curve. These day-to-day measurements suggest that DESI-MS can be successfully employed for routine quantitative analysis. The use of the analog atenolol as an internal standard and further considerations that should improve quantitation by DESI-MS are also presented. Copyright (C) 2008 John Wiley & Sons, Ltd.