Hard-food mastication suppresses complete FREUND's adjuvant-induced nociception
Hard-food mastication suppresses complete FREUND's adjuvant-induced nociception
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DOI：
10.1016/s0306-4522(03)00214-8
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发表时间：
2003-09
期刊：
影响因子：
3.3
通讯作者：
A. Ogawa;T. Morimoto;J. Hu;Y. Tsuboi;A. Tashiro;K. Noguchi;H. Nakagawa;K. Iwata
中科院分区：
文献类型：
--
作者：
A. Ogawa;T. Morimoto;J. Hu;Y. Tsuboi;A. Tashiro;K. Noguchi;H. Nakagawa;K. Iwata
通过分析完全弗氏佐剂 (CFA) 诱导的伤害行为和 Fos 表达，研究咀嚼过程中食物硬度对脊髓伤害性传递的影响。行为研究表明，给定食物硬度从软变硬后，后爪注射 CFA 后，退缩潜伏期的缩短受到抑制。静脉注射后，吃硬食物的大鼠伤害行为的抑制得到了逆转。注射纳洛酮。皮下注射后，Fos 蛋白样免疫反应细胞（Fos 蛋白-LI 细胞）在 L4-6 脊髓背角的浅层和深层表达。在软食咀嚼过程中将 CFA 注射到后爪中。咀嚼硬食后咀嚼软食的大鼠中 Fos 蛋白-LI 细胞数量减少。食物硬度变化后 Fos 蛋白-LI 细胞的减少在静脉注射后逆转。纳洛酮的应用。此外，双侧下牙槽神经横断或双侧体感皮层消融后，进食硬质食物后Fos蛋白-LI细胞的抑制显着受到抑制。这些发现表明，咀嚼过程中食物硬度的变化可能会驱动阿片类药物下行系统通过三叉神经感觉通路和体感皮层，从而对慢性疼痛产生抗伤害作用。然而，IAN横断和皮质消融并没有诱导Fos表达100%逆转，这表明三叉神经感觉系统以外的系统可能参与了这种现象，例如通过脑干网状形成的通路。
The effect of food hardness during mastication on nociceptive transmission in the spinal cord was studied by analyzing complete Freund's adjuvant (CFA) induced nocifensive behavior and Fos expression. The behavioral study showed that the shortening of the withdrawal latency following CFA injection into the hind paw was depressed after a change in the given food hardness from soft to hard. The depression of nocifensive behavior in the rats with hard food was reversed after i.v. injection of naloxone. Fos protein-like immunoreactive cells (Fos protein-LI cells) were expressed in the superficial and deep laminae of the L4–6 spinal dorsal horn after s.c. injection of CFA into the hind paw during soft food mastication. The number of Fos protein-LI cells was decreased in the rats with hard food mastication followed by soft food. This reduction of Fos protein-LI cells following change in food hardness was reversed after i.v. application of naloxone. Furthermore, the depression of Fos protein-LI cells following hard food intake was significantly inhibited after bilateral inferior alveolar nerve transection or bilateral ablation of the somatosensory cortex. These findings suggest that the change in food hardness during mastication might drive an opioid descending system through the trigeminal sensory pathway and somatosensory cortex resulting in an antinociceptive effect on chronic pain. However, IAN transection and cortical ablation did not induce 100% reversal of Fos expression, suggesting other than trigeminal sensory system may be involved in this phenomena, such as the pathway through the brainstem reticular formation.