Triple helical collagen-like peptides: Engineering and applications in matrix biology
Triple helical collagen-like peptides: Engineering and applications in matrix biology
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DOI：
10.1080/03008200591008518
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发表时间：
2005-01-01
影响因子：
2.9
通讯作者：
Koide, T
中科院分区：
文献类型：
--
作者：
Koide, T
胶原蛋白是一种具有三螺旋结构的大型不溶性蛋白质，是细胞外基质最重要的组成部分。胶原蛋白的功能并不局限于作为一种结构蛋白为各种组织和器官提供机械强度，因为已经指出胶原蛋白通过与其他大分子的特定相互作用而表现出各种生物学功能。然而，使用天然三螺旋胶原蛋白往往是麻烦的，因为它的不溶性和凝胶性。相反，人们设计了三螺旋胶原样肽，并将其用作胶原替代品，用于胶原结构、稳定性和生物功能（包括与其他蛋白质和培养细胞的结合）的研究。本文综述了肽的设计、合成以及胶原样肽在当前基质生物学中的应用进展，同时强调了基于肽的策略的优势。
Collagen, a large insoluble protein with a characteristic triple helical structure, is found as the most prominent component of extracellular matrix. The functions of collagen are not limited to providing mechanical strength to various tissues and organs as a structural protein, as it has been pointed out that collagen exhibits various biological functions through specific interactions with other macromolecules. However, the use of native triple helical collagen is often troublesome because of its insolubility and gelating properties. Instead, triple helical collagen-like peptides have been designed and are used as collagen surrogates in studies on collagen structure, stability, and biological functions including binding to other proteins and cultured cells. This article reviews recent progress in peptide design, synthesis, and the applications of collagen-like peptides in current matrix biology, while emphasizing the advantages of the peptide-based strategy.