Deamidation of a model hexapeptide in poly(vinyl alcohol) hydrogels and xerogels.
Deamidation of a model hexapeptide in poly(vinyl alcohol) hydrogels and xerogels.
复制标题
聚（乙烯醇）水凝胶和干凝胶中模型六肽的脱酰胺作用。
DOI：
10.1034/j.1399-3011.2000.00156.x
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Topp,EM
中科院分区：
文献类型：
--
作者：
Lai,MC;Schowen,RL;Borchardt,RT;Topp,EM
Polymeric controlled release systems have been proposed to prolong the half‐lives of protein and peptide drugsin vivoand to deliver active drug at a controlled rate. These systems are ineffective, however, if the drug is not stable during storage and release. This study addresses the effect of poly(vinyl alcohol) on the stability and release of an incorporated hexapeptide, VYPNGA, which undergoes deamidation. Two types of peptide‐loaded poly(vinyl alcohol) matrices were formed, a semisolid hydrogel and a lower water content ‘xerogel’, and stored at 50°C for up to 122 days. The hexapeptide was less stable in both poly(vinyl alcohol) matrices than in aqueous buffer or lyophilized polymer‐free powders. The type of poly(vinyl alcohol) matrix appeared to influence the degradation mechanism, since the product distributions differ in the hydrogel and the xerogel. The results suggest that, rather than stabilizing this peptide, incorporation in poly(vinyl alcohol) matrices reduces stability relative to solution and lyophilized controls.
