Rejuvenation of periosteal chondrogenesis using local growth factor injection.
Rejuvenation of periosteal chondrogenesis using local growth factor injection.
复制标题
使用局部生长因子注射恢复骨膜软骨形成。
DOI：
10.1016/j.joca.2008.10.011
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发表时间：
2009
影响因子：
7
通讯作者：
O'Driscoll,SW
中科院分区：
文献类型：
--
作者：
Reinholz,GG;Fitzsimmons,JS;Casper,ME;Ruesink,TJ;Chung,HW;Schagemann,JC;O'Driscoll,SW
OBJECTIVETo examine the potential for rejuvenation of aged periosteum by local injection of transforming growth factor-beta1 (TGF-β1) and insulin-like growth factor-1 (IGF-1) alone or in combination to induce cambium cell proliferation and enhance in vitro periosteal cartilage formation.METHODSA total of 367 New Zealand white rabbits (6, 12, and 24+ month-old) received subperiosteal injections of TGF-β1 and/or IGF-1 percutaneously. After 1, 3, 5, or 7 days, the rabbits were sacrificed and cambium cellularity or in vitro cartilage forming capacity was determined.RESULTSA significant increase in cambium cellularity and thickness, and in vitro cartilage formation was observed after injection of TGF-β1 alone or in combination with IGF-1. In 12 month-old rabbits, mean cambium cellularity increased 5-fold from 49 to 237cells/mm and in vitro cartilage production increased 12-fold from 0.8 to 9.7mg 7 days after TGF-β1 (200ng) injection compared to vehicle controls (P<0.0001). A correlation was observed between cambium cellularity and in vitro cartilage production (R2=0.98). An added benefit of IGF-1 plus TGF-β1 on in vitro cartilage production compared to TGF-β1 alone was observed in the 2 year-old rabbits. IGF-1 alone generally had no effect on either cambium cellularity or in vitro cartilage production in any of the age groups.CONCLUSIONSThese results clearly demonstrate that it is possible to increase cambium cellularity and in vitro cartilage production in aged rabbit periosteum, to levels comparable to younger rabbits, using local injection of TGF-β1 alone or in combination with IGF-1, thereby rejuvenating aged periosteum.
