Synaptic Specializations of Melanopsin-Retinal Ganglion Cells in Multiple Brain Regions Revealed by Genetic Label for Light and Electron Microscopy
Synaptic Specializations of Melanopsin-Retinal Ganglion Cells in Multiple Brain Regions Revealed by Genetic Label for Light and Electron Microscopy
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DOI：
10.1016/j.celrep.2019.09.006
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发表时间：
2019-10-15
期刊：
影响因子：
8.8
通讯作者：
Panda, Satchidananda
中科院分区：
文献类型：
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作者：
Kim, Keun-Young;Rios, Luis C.;Panda, Satchidananda
使用一种新的膜靶向版的相关光电子显微镜（CLEM）遗传探针，研究了表达黑视素的视网膜神经节细胞（也称为内在光敏视网膜神经节细胞（ipRGCs））的形式和突触精细结构。ipRGCs投射到多个脑区，由于该方法标记了整个神经元，因此可以分析多个视网膜受体脑区的神经末梢，包括视交叉上核（SCN）、橄榄前核（OPN）和外侧展状亚区。尽管ipRGC为OPN和SCN提供了唯一的直接视网膜输入，但在每个目标区域中，ipRGC末端的枢纽和扣被发现有显著差异。在SCN中还发现了一个树突-树突化学突触（ddcs）网络，ipRGC轴突终端优先在ddcs连接的细胞上突触。为实现这一分析而开发的方法应该会推动其他高分辨率长距离脑回路的CLEM研究。
The form and synaptic fine structure of melanopsin-expressing retinal ganglion cells, also called intrinsically photosensitive retinal ganglion cells (ipRGCs), were determined using a new membrane-targeted version of a genetic probe for correlated light and electron microscopy (CLEM). ipRGCs project to multiple brain regions, and because the method labels the entire neuron, it was possible to analyze nerve terminals in multiple retinorecipient brain regions, including the suprachiasmatic nucleus (SCN), olivary pretectal nucleus (OPN), and subregions of the lateral geniculate. Although ipRGCs provide the only direct retinal input to the OPN and SCN, ipRGC terminal arbors and boutons were found to be remarkably different in each target region. A network of dendro-dendritic chemical synapses (DDCSs) was also revealed in the SCN, with ipRGC axon terminals preferentially synapsing on the DDCS-linked cells. The methods developed to enable this analysis should propel other CLEM studies of long-distance brain circuits at high resolution.