Whn and mHa3 are components of the genetic hierarchy controlling follicle differentiation
Whn and mHa3 are components of the genetic hierarchy controlling follicle differentiation
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DOI：
10.1016/s0925-4773(99)00218-x
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发表时间：
1999-12-01
影响因子：
2.6
通讯作者：
Boehm, T
中科院分区：
文献类型：
--
作者：
Meier, N;Dear, TN;Boehm, T
携带有缺陷的Whn转录因子基因的裸鼠特征性毛发生长障碍的分子基础尚不清楚。通过代表性差异分析比较野生型和裸鼠背部皮肤的 mRNA 群体，发现突变型小鼠中不存在酸性毛发角蛋白基因 3 (mHa3) mRNA。 Whn 和酸性毛发角蛋白基因在毛囊、指甲形成区域和舌头的丝状乳头中共表达：mHa3 基因的表达通常在 Whn mRNA 约 1 天后可检测到，在其不存在时迅速停止。 Whn 在毛发周期的生长期（生长）阶段在基质、皮层和外根鞘中强烈表达；其表达在退行期迅速下降，并且在休止期无法检测到。在裸鼠中，指甲和胡须毛囊中维持低水平的 mHa3 表达，而皮毛毛囊和丝状乳头中完全不表达。因此，裸表型代表了遗传性皮肤病的第一个例子，这种疾病与角蛋白基因的表达缺失而不是结构突变有关。毛囊和胡须毛囊之间明显的分子差异与裸鼠触须机械稳定性的改善相关，这表明 mHa3 是毛干的重要结构成分。 (C) 1999 Elsevier Science Ireland Ltd. 保留所有权利。
The molecular basis of the characteristic hair growth disorder in nude mice that carry a defective Whn transcription factor gene is unknown. A comparison of mRNA populations from wild-type and nude mice back skin by representational difference analysis revealed the absence of acidic hair keratin gene 3 (mHa3) mRNA in mutant mice. Whn and acidic hair keratin genes are co-expressed in hair follicles, nail forming regions and filiform papillae of the tongue: expression of the mHa3 gene is generally detectable about 1 day after Whn mRNA and rapidly ceases in its absence. Whn is strongly expressed during the anagen (growth) phase of the hair cycle in matrix, cortex and outer root sheath; its expression rapidly declines during catagen and is undetectable in telogen phases. In nude mice, low levels of mHa3 expression are maintained in nails and whisker follicles, whereas expression is completely absent in pelage hair follicles and filiform papillae. Thus, the nude phenotype represents the first example of an inherited skin disorder that is associated with the loss of expression rather than structural mutation of keratin genes. The distinct molecular difference between pelage and whisker follicles correlates with the improved mechanical stability of vibrissae in nude mice, implicating mHa3 as an important structural component of the hair shaft. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.