Scintillation counting of 32P without added scintillator in aqueous solutions and organic solvents and on dry chromatographic media.
Scintillation counting of 32P without added scintillator in aqueous solutions and organic solvents and on dry chromatographic media.
复制标题
不添加闪烁剂的 32P 在水溶液和有机溶剂中以及在干色谱介质上的闪烁计数。
DOI：
10.1016/0003-2697(70)90303-9
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发表时间：
1970
影响因子：
2.9
通讯作者：
L. L. Bieber
中科院分区：
文献类型：
--
作者：
R. T. Haviland;L. L. Bieber
由32 P产生的切伦科夫辐射使得利用常规闪烁计数器测定几乎任何样品中的32 P成为可能。有机溶剂提供高的绝对效率-甲苯的效率最高，约为80%。当存在强酸、碱或盐时，在水溶液中获得约50%的绝对效率，变化非常小。纸和硅胶在没有液体存在的情况下具有类似的效率，因为切伦科夫辐射发生在聚乙烯和玻璃中。样品完全不受计数的影响。所有样品的计数条件均保持不变。效率随体积、溶液组成和小瓶材料而变化。已使用快速、高精度的方法测定了各种水溶液和有机溶剂相对于水的有效性。
Čerenkov radiation produced by32P makes possible a convenient and efficient method for determination of32P in virtually any sample utilizing conventional scintillation counters. Organic solvents provide high absolute efficiencies—the highest efficiency measured, approximately 80%, was obtained with toluene. Approximately 50% absolute efficiency is obtained in aqueous solutions with very little variation when strong acids, bases, or salts are present. Paper and silica gel give similar efficiencies with no liquid present because Čerenkov radiation occurs in polyethylene and in glass. Samples are completely unaltered by the counting. Counting conditions are constant for all samples. The efficiency varies with volume, solution composition, and vial material. Efficiencies relative to water for a wide variety of aqueous solutions and organic solvents have been determined using a rapid, highly accurate method.