Different expression profiles of jasmonic acid and salicylic acid inducible genes in the tomato plant against herbivores with various feeding modes
Different expression profiles of jasmonic acid and salicylic acid inducible genes in the tomato plant against herbivores with various feeding modes
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DOI：
10.1007/s11829-011-9174-z
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发表时间：
2012-06-01
影响因子：
1.6
通讯作者：
Mitsuhara, Ichiro
中科院分区：
文献类型：
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作者：
Kawazu, Kei;Mochizuki, Atsushi;Mitsuhara, Ichiro
我们比较了番茄（Solanum lycopersicum cv. Micro-Tom）中茉莉酸（JA）诱导基因（Pin2 和 LapA1）和水杨酸（SA）诱导基因（PRb-1b 和 GluB）与不同饲喂模式的食草动物的表达谱：斜纹夜蛾和甜菜夜蛾的咀嚼叶幼虫；西花蓟马 (Frankliniella occidentalis) 和二斑叶螨 (Tetranychus urticae) 作为细胞内容物的饲养者；和潜叶蝇（Liriomyza sativae）。斜纹夜蛾和甜菜夜蛾幼虫的摄食主要激活 JA 诱导基因，类似于对伤口刺激的反应。正如先前在拟南芥中报道的那样，蓟马 F. occidentalis 的取食也会激活 JA 诱导基因。叶螨T. urticae的摄食激活了JA诱导的LapA1基因，但没有激活JA诱导的Pin2基因，并且另外激活了SA诱导的基因，这伴随着SA的积累。这可能是抑制 JA 信号通路诱导的菌株。潜叶蝇 L. sativae 产卵一天后，JA 诱导基因被激活。然而，当苜蓿幼虫孵化并开始在叶组织内进食后，JA 诱导型基因表达下降，而 SA 诱导型基因表达增加。苜蓿幼虫摄食对 SA 诱导基因（PRb-1b 和 GluB）的激活似乎抑制了 JA 介导的植物防御，但似乎与 SA 积累无关。
We compared the expression profiles of jasmonic acid (JA)-inducible genes (Pin2 and LapA1) and salicylic acid (SA)-inducible genes (PRb-1b and GluB) in the tomato (Solanum lycopersicum cv. Micro-Tom) against herbivores using differing feeding modes: the leaf-chewing larvae of the insects Spodoptera litura and S. exigua; the western flower thrips (Frankliniella occidentalis) and the two-spotted spider mite (Tetranychus urticae) as cell-content feeders; and the leaf miner fly (Liriomyza sativae). Feeding by larvae of both S. litura and S. exigua chiefly activated JA-inducible genes, similar to the response to wound stimuli. Feeding by the thrips F. occidentalis also activated JA-inducible genes, as previously reported in Arabidopsis. Feeding by the spider mite T. urticae activated a JA-inducible LapA1 gene but did not activate a JA-inducible Pin2 gene and additionally activated SA-inducible genes, which were accompanied by the accumulation of SA. This may be a strain that represses induction of the JA signaling pathway. One day after oviposition by the leaf miner fly, L. sativae, JA-inducible genes were activated. However, after the L. sativae larvae hatched and began eating within the leaf tissues, JA-inducible gene expression decreased and SA-inducible gene expression increased. Activation of SA-inducible genes (PRb-1b and GluB) by L. sativae larval feeding seems to suppress JA-mediated plant defense but appears to be unrelated to SA accumulation.