AFFINITY OF THE GASTRIC PATHOGEN HELICOBACTER-PYLORI FOR THE N-SULFATED GLYCOSAMINOGLYCAN HEPARAN-SULFATE
AFFINITY OF THE GASTRIC PATHOGEN HELICOBACTER-PYLORI FOR THE N-SULFATED GLYCOSAMINOGLYCAN HEPARAN-SULFATE
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DOI：
10.1099/00222615-38-4-240
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发表时间：
1993-04-01
影响因子：
3
通讯作者：
WADSTROM, T
中科院分区：
文献类型：
--
作者：
ASCENCIO, F;FRANSSON, LA;WADSTROM, T
结合I-125-硫酸乙酰肝素是从胃十二指肠溃疡疾病患者中分离的幽门螺杆菌菌株的共同特性。结合是（i）可饱和的;（ii）通过加入未标记的硫酸乙酰肝素和肝素可逆;（iii）通过未标记的硫酸乙酰肝素、肝素和肝素寡糖抑制，但不被其他大小相当的糖胺聚糖抑制（硫酸软骨素和硫酸皮肤素）或高度糖基化的糖蛋白（猪胃粘蛋白和胎球蛋白）;（iv）通过热处理减少（80 ℃，10分钟），并将细菌暴露于链霉蛋白酶E、蛋白酶K、胰蛋白酶和胰凝乳蛋白酶，但不受胃蛋白酶和神经氨酸酶处理的影响;和（v）时间依赖性、pH依赖性和离子强度依赖性。结合数据的Scatchard图分析表明存在一类硫酸乙酰肝素高亲和力受体（K（d）= 9 × 10（-9）M）。
Binding of I-125-heparan sulphate was a common property of Helicobacter pylori strains isolated from patients with gastroduodenal ulcer diseases. Binding was (i) saturable; (ii) reversible by the addition of unlabelled heparan sulphate and heparin; (iii) inhibited by unlabelled heparan sulphate, heparin, and heparin oligosaccharides but not by other glycosaminoglycans of comparable size (chondroitin sulphate and dermatan sulphate) or by highly glycosylated glycoproteins (hog gastric mucin and fetuin); (iv) reduced by heat treatment (80-degrees-C, 10 min) and exposure of the bacteria to pronase E, proteinase K, trypsin and chymotrypsin, but unaffected by treatment with pepsin and neuraminidase; and (v) time-, pH-, and ionic strength-dependent. Scatchard plot analysis of the binding data indicated the presence of one class of high-affinity receptor (K(d) = 9 x 10(-9) M) for heparan sulphate.