Biphasic Effect of ATP on In Vitro Mineralization of Dental Pulp Cells
Biphasic Effect of ATP on In Vitro Mineralization of Dental Pulp Cells
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DOI：
10.1002/jcb.26206
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发表时间：
2018-01
影响因子：
4
通讯作者：
Oranuch Techatharatip;N. Nowwarote;S. Taebunpakul;P. Pavasant
中科院分区：
文献类型：
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作者：
Oranuch Techatharatip;N. Nowwarote;S. Taebunpakul;P. Pavasant
牙髓细胞释放三磷酸腺苷（ATP）响应于牙髓内压力，释放的量取决于压力的大小。ATP调节干细胞向脂肪细胞和成骨细胞的分化。然而，它是未知的细胞外ATP是否影响干细胞从人类脱落乳牙（SHEDs）的干性和成骨分化。因此，本研究探讨了低浓度（0.1 μM）和高浓度（10 μM）的细胞外ATP对SHED的干性和成骨分化的影响。将细胞培养在含或不含0.1-10 μM ATP的生长培养基或成骨培养基中。在生长培养基中，两种浓度的ATP都增加了多能性和成骨标志物的mRNA表达。相反，在成骨培养基中，0.1 μM ATP促进体外矿化，而10 μM ATP抑制该过程。此外，10 μM ATP刺激外核苷酸焦磷酸酶/磷酸二酯酶（ENPP）的mRNA表达和活性，ENPP是一种调节磷酸/焦磷酸比例的酶。因此，根据生长条件和浓度，ATP刺激干和体外矿化或抑制矿化。在生长培养基中，两种ATP浓度刺激多能性和成骨标志物基因表达。然而，在成骨培养基中，发现在体外矿化的双相效应;低浓度刺激，而高浓度抑制，矿化。我们建议，ATP释放由于机械应力调制的干性和分化的SHEDs。J.细胞。119：488-498，2018.© 2017 Wiley Periodicals，Inc.
Dental pulp cells release adenosine triphosphate (ATP) in response to intrapulpal pressure and the amount released depends on the magnitude of the pressure. ATP regulates the differentiation of stem cells into adipocytes and osteoblasts. However, it is unknown whether extracellular ATP influences the stemness and osteogenic differentiation of stem cells from human exfoliated deciduous teeth (SHEDs). Therefore, this study investigated the effects of extracellular ATP at a low (0.1 μM) and high (10 μM) concentration on the stemness and osteogenic differentiation of SHEDs. Cells were cultured in either growth medium or osteogenic medium with or without 0.1–10 μM ATP. In growth medium, both concentrations of ATP increased the mRNA expression of pluripotent and osteogenic markers. In contrast, in osteogenic medium, 0.1 μM ATP enhanced in vitro mineralization, whereas 10 μM ATP inhibited this process. In addition, 10 μM ATP stimulated the mRNA expression and activity of ectonucleotide pyrophosphatase/phosphodiesterase (ENPP), an enzyme that regulates the phosphate/pyrophosphate ratio. Thus, depending on the growth condition and its concentration, ATP stimulated stemness and in vitro mineralization or inhibited mineralization. In growth medium, both ATP concentrations stimulated pluripotent and osteogenic marker gene expression. However, in osteogenic medium, a biphasic effect was found on in vitro mineralization; the low concentration stimulated, whereas the high concentration inhibited, mineralization. We propose that ATP released due to mechanical stress modulates the stemness and differentiation of SHEDs. J. Cell. Biochem. 119: 488–498, 2018. © 2017 Wiley Periodicals, Inc.